高效液相色谱法测定虎杖中白藜芦醇的含量  

Determination of Resveratrol in Polygonum cuspidatum by HPLC

作　　者：夏海武[1] 谭志静[1] 苗永美[1] 邓盾[1] 

机构地区：[1]安徽科技学院,安徽凤阳233100

出　　处：《湖北农业科学》2011年第4期825-827,共3页Hubei Agricultural Sciences

基　　金：安徽科技学院人才专项(ZRC200692)

摘　　要：采用高效液相色谱法测定了虎杖不同生长条件及不同部位的白藜芦醇含量。建立了以色谱柱为Zorbax CB-C18(4.6×250 mm,5μm),流动相为甲醇-水(35∶65,V/V),流速为0.8 mL/min,检测波长为306 nm,柱温为室温,进样量为80μL的高效液相色谱方法。结果表明,白藜芦醇浓度在2～100μg/mL范围内与峰面积积分值呈良好线性关系(r=0.999 9);平均回收率为99.1%,RSD=1.2%(n=6)。野生虎杖和组织培养虎杖白藜芦醇含量存在较大差异,虎杖不同部位白藜芦醇含量也存在较大差异,其中以野生虎杖根中白藜芦醇含量最高。虽然在不同培养条件下,虎杖的不同部位都含有白藜芦醇,但与野生虎杖根相比含量差距较大,不能代替野生虎杖根的药用价值。The contents of resveratrol in different growing condition and different part of Polygonum cuspidatum were detected by HPLC.The separation was performed on Zorbax CB-C18 column（250 mm×4.6 mm,5 μm） in methanol-water（V/V=35/65）with the flow rate of 0.8 mL/min and detected at 306 nm in room temperature after injecting 80 μL sample.The linear range of resveratrol was 2～100 μg/mL with an average recovery of 99.1%（RSD=1.2%,n=6）.The content of resveratrol in wild and tissue culture of Polygonum cuspidatum were different,and the contents of resveratrol in different parts of Polygonum cuspidatum also varied.The highest resveratrol in Polygonum cuspidatum was observed in wild plants.Though resveratrol of Polygonum cuspidatum differed in different culture conditions,the highest resveratrol content was detected in the roots of wild Polygonum cuspidatum,which indicated that the medical value of wild Polygonum cuspidatum was irreplaceable.

关 键 词：虎杖 白藜芦醇 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]