镉长期暴露对中华稻蝗抗氧化机制的影响  

Activity of Antioxidant Enzymes in Response to Cadmium in Oxya Chinensis

作　　者：李丽君[1,2] 郭亚平[3] 刘平[2] 王跃[1] 马恩波[1] 

机构地区：[1]山西大学应用生物学研究所,太原030006 [2]山西省农业科学院农业环境与资源研究所,太原030006 [3]山西大学生命科学学院,太原030006

出　　处：《农业环境科学学报》2011年第3期443-448,共6页Journal of Agro-Environment Science

基　　金：国家自然科学重大国际合作项目(30810103907);山西省留学人员管理委员会基金(2008114);山西省农业科学院博士基金(YBSJJ0702)

摘　　要：采用生长于Cd2+污染条件下的小麦苗喂养中华稻蝗的慢性染毒方法,研究了Cd2+胁迫对中华稻蝗SOD、CAT、GPx活性和H2O2浓度的影响。结果表明,在Cd2+的作用下,中华稻蝗体内SOD、CAT、GPx活性和H2O2浓度均发生变化,表明Cd2+可导致中华稻蝗体内产生ROS。SOD、CAT、GPx3种酶对Cd2+有一定的耐受性:当Cd2+浓度在这3种酶的耐受范围内,酶活性提高,反之酶活性降低。Cd2+与抗氧化系统间的关系复杂,SOD、CAT、GPx共同承担清除ROS、保护中华稻蝗的重要作用。This paper was to present the influence of Cd on antioxidant enzymes in Oxya chinensis.The activities of superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GPx）,and the level of hydrogen peroxide（H2O2） were determined in O.chinensis which were fed for 30 d on wheat seedlings exposed to different Cd concentration（0.25（control）,24.57,36.67,43.50 mg·kg-1 and 50.70 mg·kg-1）.The results indicated that these enzymes activities and H2O2 level were varied in the presence of Cd,which suggested that there was an overproduction of reactive oxygen species（ROS） in O.chinensis following Cd treatment.In males,SOD activity declined by 19.86%,38.65%,28.98% and 71.49% respecting to control value,respectively,along with a decrease in CAT activity by 6.90%,68.70%,76.49% and 71.04% at 24.57,36.67,43.50 mg·kg-1 and 50.70 mg·kg-1 Cd in wheat seedlings,respectively.GPx activity increased by 56.73%,102.95%,70.17% at 24.57,36.67,43.50 mg·kg-1 Cd,respectively,decreased by 11.08% at 50.70 mg·kg-1 Cd,and H2O2 level increased by 13.61%,13.69% and 102.47% at 24.57,43.50 mg·kg-1 and 50.70 mg·kg-1 Cd,respectively,and declined by 27.05% at 36.67 mg·kg-1 Cd.In females,SOD activity increased by 64.29% and 23.29% at 24.57 mg·kg-1 and 50.70 mg·kg-1 Cd,decreased by 31.78% and 18.54% at 36.67 mg·kg-1 and 43.50 mg·kg-1 Cd.CAT activity declined by 8.73%,45.31%,9.98% and 5.17% at 24.57,36.67,43.50 mg·kg-1 and 50.70 mg·kg-1 Cd,respectively.GPx activity increased by 0.15% and 0.88% at 24.57 mg·kg-1 and 43.50 mg·kg-1 Cd,decreased by 20.50% and 44.40% at 36.67 mg·kg-1 and 50.70 mg·kg-1 Cd.H2O2 level elevated by 42.68%,and 26.01 % at 24.57 mg·kg-1 and 43.50 mg·kg-1,reduced by 15.10% and 13.97% at 36.67 mg·kg-1 and 50.70 mg·kg-1 Cd.The relationship between Cd toxicity and antioxidative system was complex,and antioxidant enzymes,such as SOD,CAT and GPx were involved in the mechanism to protect O.chinensis from ROS induced by Cd.Meanwhile,SOD,CAT and GPx played significant roles in cell defense agains

关 键 词：中华稻蝗(Oxya chinensis) 镉(CD) 超氧化物歧化酶(SOD) 过氧化氢酶(CAT) 谷胱甘肽过氧化物酶(GPx) 

分 类 号：X503.223[环境科学与工程—环境工程]