茯苓多糖的纯化及分子量测定  

Purification of Tuckahoe Polysaccharide and Determination of Its Molecular Weight

作　　者：颜军[1] 陶涛[1] 孙晓春[2] 何钢[1] 易勇[1] 苟小军[1,2] 

机构地区：[1]成都大学药食同源植物资源开发重点实验室,四川成都610106 [2]四川抗菌素工业研究所,四川成都610052

出　　处：《化学与生物工程》2011年第3期88-90,94,共4页Chemistry & Bioengineering

基　　金：四川省中医药管理局科技专项项目(2008-01);四川省教育厅科研资助项目(08ZA173)

摘　　要：通过水提醇沉的方法得到茯苓粗多糖,精制后采用离子交换柱层析对多糖进行分离纯化,用高效凝胶过滤色谱法(HPGFC)测定多糖分子量。结果表明,茯苓粗多糖用Sevag法脱蛋白效果较好,获得了精制茯苓多糖(TAP);经DEAE-650C层析柱纯化,得到中性多糖(TAP1)和酸性多糖(TAP2),高效凝胶过滤色谱法测定其分子量分别为11 721和44 065。Tuckahoe polysaccharide was extracted by boiling water and precipitated by alcohol,and purified using ion-exchange column chromatography,and its molecular weight was determined by high performance gel filtration chromatography（HPGFC）.The results showed that Sevag method was effective for removing proteins from crude polysaccharide to obtain refined tuckahoe polysaccharide（TAP）;two components,neutral polysaccharide TAP1 and acidic polysaccharide TAP2,were got through DEAE-650C column chromatography purification,whose molecular weight were determined by HPGFC as 11 721 and 44 065,respectively.

关 键 词：茯苓多糖 纯化 分子量 

分 类 号：Q539.1[生物学—生物化学] O636.9[理学—高分子化学]