真性红细胞增多症患者T细胞的克隆特点  

The feature of T cell clonality with polycythemia vera

作　　者：李扬秋[1] 杨力建[1] 陈少华[1] 乔旭刚[1] 祁明芳[2] 许敏华[2] 罗更新[2] 朱平[3] 

机构地区：[1]暨南大学血液病研究室,广州510632 [2]暨南大学第一附属医院血液科 [3]北京医科大学第一医院血液病研究室

出　　处：《中华血液学杂志》1999年第11期570-572,共3页Chinese Journal of Hematology

摘　　要：目的　了解真性红细胞增多症（ＰＶ）患者外周血Ｔ细胞受体（ＴＣＲ）Ｖβ２４ 个亚家族基因的表达及其克隆特点。方法　采用逆转录多聚酶链反应（ＲＴＰＣＲ） 检测３ 例ＰＶ 患者外周血单个核细胞ＴＣＲＶβ２４ 个亚家族基因表达情况；采用基因扫描分析Ｔ细胞的克隆特点。结果　３ 例ＰＶ患者外周血仅表达４ ～１４ 个Ｖβ亚家族，主要为Ｖβ２ 、Ｖβ３ 、Ｖβ１６ 、Ｖβ２１ 和Ｖβ２３ ；２ 例患者表达的Ｖβ３ 和Ｖβ２３Ｔ细胞为克隆性增殖。结论　ＰＶ 患者外周血ＴＣＲＶβ亚家族的优势表达和克隆性增殖可能是患者体内对抗恶性细胞的免疫反应特征。Objective To investigate the expression and clonality of TCR Vβ subfamily genes in the T cells in polycythemia vera. Methods The 24 genes of the TCR Vβ subfamily were amplified with peripheral blood mononuclear cells from 3 cases of polycythemia vera(PV) by RT PCR, then the PCR products were further analyzed by genescan technique to evaluating the clonality of the detectable TCR Vβ T cells. Results Only 4～14 of the 24 Vβ subfamily T cells were found in the 3 PV cases, the commonly expressed Vβ subfamily genes were Vβ 2, Vβ 3, Vβ 16 , Vβ 21 and Vβ 23 . Two clonal T cells of Vβ 3 and Vβ 23 were identified in 2 cases. Conclusions The skew distribution and clonal expansion of TCR Vβ subfamily T cells in patients with polycythemia vera might be a feature of the immune response for malignant cells.

关 键 词：受体 抗原 T细胞 红细胞增多症 真性 

分 类 号：R555.1[医药卫生—血液循环系统疾病]