枯草芽孢杆菌淀粉酶高产菌株的紫外诱变研究  

Mutation of Bacillus subtilis with High-yield of Amylase

作　　者：马颖辉[1] 曹龙奎[1] 李玉秋[2] 高岩[3] 王景会[2,3] 

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆163319 [2]吉林省农业科学院,长春130033 [3]吉林农业大学,长春130175

出　　处：《农产食品科技》2011年第1期23-26,共4页

基　　金：吉林省科学技术厅资助项目（20090248）.

摘　　要：以实验室保存的枯草芽孢杆菌为出发菌株，利用紫外诱变的方法，以获取可高效发酵生产淀粉酶的突变株。通过筛选及连续传代实验，确定了一株突变性状能稳定遗传的突变株，其淀粉酶产量达到2．264U／mL，比出发菌株提高了190．63％。In order to increase the output of amylase, the original Bacillus strain preserved by our laboratory was mutated using UV mutagenesis. One mutant strain, which was stable in hereditary, was obtained by screening. The yield of amylase by the mutated strain reached 2.264 U/mL, which was 190.63% higher than that produced by the starting strain.

关 键 词：紫外诱变 枯草芽孢杆菌 淀粉酶 

分 类 号：O93[理学]