虎杖乙酸乙酯萃取部分抗人疱疹病毒研究  

Research about antiviral effect of ethyl acetate component of Rhizoma polygoni cuspidati on herpes virus hominis

作　　者：王志洁[1] 方学韫[2] 程仲敏[2] 

机构地区：[1]湖北医科大学病毒研究所,武汉430071 [2]湖北医科大学附属二院中医科,武汉430071

出　　处：《中国现代应用药学》1999年第5期27-31,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：湖北省卫生厅科教处资助

摘　　要：目的：ＨＳＶ 可引起多种疾病，阿昔洛韦（ＡＣＶ） 治疗ＨＳＶ 感染效果虽好，但易出现耐药毒株；新药Ｃｉｄｏｆｏｖｉｒ 对ＡＣＶ 耐药毒株效果好，毒性也大。ＨＳＶ 疫苗临床实验效果也不好。为了解决这些问题，必须继续开发新药。虎杖是一种抗病毒范围广的中药，为了了解虎杖的抗病毒性能并开发利用，我们对虎杖乙酸乙酯萃取部分的抗ＨＳＶ 药效进行了研究。方法：我们以ＡＣＶ 为阳性对照，利用病毒细胞病变抑制及空斑减数实验观察了药效，并且用中效作用原理对药效进行了分析。结果：在ＨＥｐ２ 细胞系统中，ＡＣＶ 对ＨＳＶ１Ｆ 株直接杀灭、增殖的抑制及感染阻断的ＥＤ５０ 分别为１３ ．２２ ，９ ．０９ 和８ ．７１μｇ／ｍｌ；晶Ⅳ的相应数值分别为０ ．０９ ，０ ．０７ 和０ ．０３μｇ／ｍｌ；晶Ⅳ的三个治疗指数（ＴＩ）分别为ＡＣＶＴＩ的３０７ ，２７２ 和６０９ 倍。ＡＣＶ 对ＨＳＶ２ ３３３ 株直接杀灭、增殖抑制及感染阻断的ＥＤ５０ 分别为１６ ．４５ ，１０ ．８５ 和１８ ．６２μｇ／ｍｌ；晶Ⅳ的相应数值分别为０ ．０７ ，０ ．０９ 和２ ．２９μｇ／ｍｌ；晶Ⅳ的三个ＴＩ分别为ＡＣＶＴＩ的４９８ ，２５１ 和１７ 倍。结论：晶Ⅳ抗ＨＳＶ 药效比ＡＣＶ 强，毒?OBJECTIVE:Herpes simplex virus can induce many diseases,although therapeutical effect of ACV on HSV infection is good,but resistant strains against ACV appear easily,and new drug cidofovir can cure the infection of ACV resistant strains,but it has obvious toxicity,the results of clinical trials of HSV vaccines are not good.In order to resoluve these problems,it is necessary to exploit new antiherpes drugs contineously.Polygonum cuspidati is a Chinese medicinal herb with wide antiviral range,understanding and exploiting it′s antiviral property,we observed antiherpes effects of ethylacetate extract component of it.METHODS:With ACV as positive control,we investigated antiherpes effects of ethyl acetate extract component,i.e. crystalline Ⅲ and crystalline Ⅳ,by way of CPE inhibition and plaque reduction assays,and analyzed effects with median effect principle.RESULTS:In HEp 2 cell system:the ED 50 s of direct virucidal activity,the inhibition effects on the multiplication and infection of HSV 1 F strain of ACV are 13.22μg/ml,9.09μg/ml and 8.71μg/ml respectively,the corresponding figures of crystalline Ⅳ are 0.09,0.07 and 0.03μg/ml respectively.The treatment indices of crystalline Ⅳ are 307,272,609 times of ACV TIs.The ED 50 s of diredct virucidal activity,the inhibition effects on the multiplication and infection of HSV 2 333 strain are 16.45μg/ml,10.85μg/ml and 18.62μg/ml respectively,the corresponding figures of crystalline Ⅳ are 0.07μg/ml,0.09μg/ml and 2.29μg/ml respectively.The treatment indices of crystalline Ⅳ are 498,251,17 times of ACV TIs.CONCLUSION:The antiherpes effects of crystalline Ⅳ is stronger than ACV,and the toxicity of crystalline Ⅳ is less than ACV,it should be worth exploitation.

关 键 词：虎杖 虎杖乙酸乙酯 萃取部分 抗疱疹病毒 

分 类 号：R285.5[医药卫生—中药学]