HPLC色谱法测定贵州黔北凹叶厚朴中和厚朴酚的含量  

Content Determination of Honokiol in QianBeis Magnolia officinalis by HPLC

作　　者：冯华[1] 王样培[2] 申桂霞 邹孔强 王小东[1] 

机构地区：[1]遵义市药品检验所,贵州遵义563002 [2]贵州贵阳中医学院,贵州贵阳553002

出　　处：《现代中药研究与实践》2011年第2期68-70,共3页Research and Practice on Chinese Medicines

基　　金：遵义市中药现代化科技产业研究开发专项项目课题(项目编号:市科合中药专字〔2008〕2号)

摘　　要：目的建立高效液相色谱法测定贵州黔北凹叶厚朴中和厚朴酚的含量。方法 ?色谱条件:色谱柱为Diamonsil C18,以甲醇-水(78:22)为流动相;流速为1.0 mL/min;柱温25℃;检测波长为294 nm。结果和厚朴酚线性范围为0.278 4μg^0.649 6μg,r=0.999 5;本品的平均回收率为102.12%,RSD为1.3%。结论本法简便、灵敏、准确。Objective To establish a method for the content determination of honokiol in QianBeis Magnolia officinalis by HPLC.Methods A Diamonsil C18 column was used with mobile phase of methanol-water（78 ： 22）,the detection wavelength was 294nm,the flow rate was 1.0 mL/min.Results The calibration curve showed a good linearity with the range of 0.627 2~1.164 8 μg,r=0.999 4.The average recovery was 102.12% and RSD was 1.3%.Conclusion The method is simple,sensitive and accurate.

关 键 词：高效液相色谱法 黔北凹叶厚朴 和厚朴酚 含量测定 

分 类 号：S567.11[农业科学—中草药栽培]