毛喉素在大鼠脑损伤后血脑屏障损害中的作用  

Role of forskolin in blood-brain barrier damage in rats after cerebral injury

作　　者：李良平[1] 俞方毅[1] 陈家祥[1] 许智蕾[1] 徐如祥[2] 

机构地区：[1]广州市红十字会医院神经外科,广州510220 [2]北京军区总医院神经外科,北京100000

出　　处：《中华神经医学杂志》2011年第3期232-234,共3页Chinese Journal of Neuromedicine

摘　　要：目的 探讨提高细胞内cAMP制剂毛喉素在脑损伤后血脑屏障损害中的作用。方法 雄性SD大鼠60只按随机数字表分为正常对照组、假手术组、脑损伤组和毛喉素治疗组，每组15只。后2组大鼠采用改进的自由落体硬膜外撞击方法制作脑损伤模型，治疗组大鼠于伤后0．5h腹腔注射毛喉索，伤后24h处死大鼠（处死前1h静脉注射20g／L伊文氏蓝），应用荧光显微镜观察伊文氏蓝的渗出，分光光度计恻定脑组织伊文氏蓝的含量。结果 正常对照组和假手术组大鼠损伤组织周同伊文氏蓝无明显渗出，脑损伤组伊文氏蓝大量渗出，治疗组与脑损伤组比较，伊文氏蓝渗出明显减少：脑损伤组大鼠左侧皮质、海马和右侧皮质、海马伊文氏蓝含量明显高于正常对照组和似手术组，差异有统计学意义（P〈O．05）；毛喉素治疗组左侧皮质伊文氏蓝含量明显低于脑损伤组，但仍高于正常对照组，差异有统计学意义（P〈0．05）。结论 提高细胞内cAMP制剂毛喉素能明显阻止血脑屏障开放。Objective To explore the role of forskolin, a reagent elevating the cellular content of cAMP, in blood-brain barrier （BBB） damage in rats after cerebral injury damage. Methods Sixty male SD rats were equally randomized into normal control group, sham-operated group, injured group and tbrskolin treatment group. Rat models of cerebral injury in the later 2 groups were induced by the improved device of Feeney weight-dropping. Foskolin treatment group was peritoneally administered forskolin 5 mg/kg 0.5 h after the brain injury. Twenty g/L Evens blue was given through intravenous injection 23 h after the injury; all the rats were sacrificed 24 h after the injury. The permeation of Evens blue was observed qualitatively with a fluorescence microscope and measured quantitatively with a spectrophotometer. Results No obvious permeation of Evens blue was noted in the surrounding areas of the damaged tissue of normal controls and sham-operated group; Evans blue significantly permeated in the injured group; as compared with that in the injured group, the permeation of Evens blue was significantly decreased in the forskolin treatment group （P〈0.05）, The level of Evens blue in the cortex and hippocampus of both side of the brain in the injured group was significantly higher that that in the normal controls and sham-operated group （P〈0.05）; the level of Evens blue in the cortex of the left side of the brain in the forskolin treatment group was significantly lower that that in the injured group, but obviously higher than that in the normal controls and sham-operated group （P〈0.05）. Conclusion Forskolin, the reagent elevatin~ the cellular content of cAMP,can significantly prevent BBB opening.

关 键 词：颅脑损伤 血脑屏障 毛喉素 

分 类 号：R651.15[医药卫生—外科学]