急性液化石油气中毒大鼠血清S100B和髓鞘碱性蛋白的检测及意义  

Changes of serum S100B and MBP in rats with liquid petroleum gas poisoning

作　　者：白润涛[1] 韩漫夫[1] 周艳霞[1] 程文蕾[1] 

机构地区：[1]深圳市第二人民医院神经内科,深圳518000

出　　处：《中华神经医学杂志》2011年第3期251-254,共4页Chinese Journal of Neuromedicine

基　　金：深圳市科技计划项目（201003029）

摘　　要：目的 研究急性液化石油气（LPG）中毒大鼠血清S100B和髓鞘碱性蛋白（MBP）的变化。方法 健康雄性成年SD大鼠54只按随机数字表法分为正常对照组（n=6）、20％LPG中毒组（n=24）和50％LPG中毒组（n=24）。后2组大鼠分别吸人20％、50％LPG，对照组吸人等体积的空气。在中毒1、2、3、7d时每组取6只大鼠进行神经功能缺损评分（NSS），ELISA法检测血清样本S100B和MBP的含量。结果 与正常对照组比较，20％LPG组中毒1d、50％LPG组中毒1d和2d时NSS评分均较高，差异有统计学意义（P〈0．05）；在1d和2d时，20％LPG组和50％LPG组大鼠血清S100B、MBP均高于正常对照组，且50％LPG组高于20％LPG组，差异有统计学意义（P〈0．05）；3d时50％LPG组血清S100B、MBP高于正常对照组和20％LPG组，差异有统计学意义（P〈0．05）；大鼠LPG中毒1d时NSS评分、血清S100B、MBP浓度最高，与其它时间点比较差异均有统计学意义（P〈0．0S），后随时间延长逐渐恢复。结论 LPG中毒大鼠血清S100B和MBP同时升高，提示胶质细胞参与LPG中毒对神经系统的损害。Objective To observe the changes of serum S100B and MBP in rats with liquid petroleum gas poisoning. Methods Fifty-four healthy adult male SD rats were randomized into normal control group （n=6）, 20% LPG poisoning group （n=24） and 50% LPG poisoning group （n=24）. Rat models of liquid petroleum gas poisoning were established in the later 2 groups, and controls were given the same volume of air. The rats of each group（n=6） were scored with neurological severity scale （NSS） and the blood serum was collected on the 1^st, 2^nd, 3^rd and 7^th d of poisoning, respectively. The levels of S100B and MBP were detected by euzymelinked immunosorbent assay （ELISA）. Results As compared with the scores of NSS in the normal control group, those on the 1^st d of poisoning in the 20% LPG poisoning group and those on the 1^st and 2^nd d of poisoning in the 50% LPG poisoning group were significantly higher （P〈0.05）. The levels of S100B and MBP in 20% and 50% LPG poisoning groups were higher than those in the control group on the 1^st and 2~nd d of poisoning （P〈0.05）; the levels of S100B and MBP in 50% LPG poisoning group were higher than those in 20% LPG poisoning group on the 1^st and 2^nd d of poisoning （P〈0.05）. The levels of S100B and MBP in 50% LPG poisoning group were higher than those in 20% LPG poisoning group and normal control group on the 3^rd d of poisoning （P〈0.05）. The NSS scores and the levels of S100B and MBP in rats with LPG poisoning enjoyed the highest scores or levels on the 1^st d of poisoning and those decreased after that. Conclusion The levels of S100B and MBP of rats with LPG poisoning increase, indicating that gliocytes participate in the mechanism of nervous system injury in rats with liquid petroleum gas poisoning.

关 键 词：中毒 S100B 髓鞘碱性蛋白 

分 类 号：R595.1[医药卫生—内科学]