肾上腺髓质素对大鼠肾损伤早期Ang-Ⅱ及MDA表达的影响  

Effects of extraneous adrenomedullin on Ang-Ⅱ and MDA expression in early renal trauma of rats

作　　者：王晓梅[1] 张淑琴 王芳[1] 陈丑彦[1] 孙少华[1] 

机构地区：[1]兰州大学第一医院病理科,兰州730000 [2]甘肃省卫生学校,兰州730000

出　　处：《山东大学学报（医学版）》2011年第2期71-74,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨外源性肾上腺髓质素(ADM)在大鼠肾脏急性机械性损伤早期对血管紧张素Ⅱ(Ang-Ⅱ)、丙二醛(MDA)表达的影响及对肾损伤的保护作用。方法健康成年普通级Wister大鼠104只,随机分为正常对照组(8只)、单纯创伤组(32只)、伤前给药组(32只)、伤后给药组(32只);后3组采用自由落体打击仪打击大鼠脊肋区制作肾机械性损伤模型。伤前、伤后给药组分别于打击前后10min腹腔注射ADM(0.1nmol/kg)。各组大鼠平均分为4批,分别在肾脏机械打击后1、6、12、24h处死。提取各组肾组织匀浆上清液,利用放免分析法检测Ang-Ⅱ浓度,比色法检测MDA浓度。结果 Ang-Ⅱ浓度:单纯创伤组在创伤早期(1、6h)明显高于正常对照组(P<0.05);伤前给药组1、6h,伤后给药组1h均低于同时间点单纯创伤组(P<0.05)。MDA浓度:单纯创伤组在创伤1h高于正常对照组(P<0.05);伤前给药组6h,伤后给药组12h均低于同时间点单纯创伤组(P<0.05)。结论外源性ADM通过降低Ang-Ⅱ与MDA的浓度而对急性创伤肾组织发挥保护作用,其中创伤前注射ADM对肾组织损伤的抑制作用优于创伤后注射ADM。Objective：To investigate the effects of extraneous adrenomedullin（ADM） on Ang-Ⅱand MDA expression,and the protective action of ADM in early mechanical renal trauma.Methods：104 healthy adult plain grade Wister rats were randomly divided into four groups： 8 in the control group,32 in the trauma group,32 in the group in which ADM was injected before trauma,and 32 in the group in which ADM was administered after the trauma.The model of mechanical kidney trauma was established by striking the skin area exactly above the kidney with a free falling ferrous hammer.In the latter two groups,ADM（0.1 nmol/kg） was injected intraperitoneally 10 minutes before or after the striking.All rats were executed in batches at the 1st,6th,12th,24th hour after the trauma.The supernatant of renal homogenates was collected and the concentrations of Ang-II and MDA were detected by radioimmunoassay or colorimetry,respectively.Results： 1） Concentration level of Ang-II： The concentration of Ang-II in the trauma group was significantly higher than that in the control group in the early trauma（1st,6th hr）（P0.05）.Also,both the concentrations of Ang-II in the group injected with ADM before trauma（1st,6th hr） and the group injected with ADM 1hr after trauma at were lower than that in the trauma group（P0.05）.2） Concentration level of MDA： The concentration of MDA in the trauma group was obviously higher than that in the control group at 1 h（P0.05）.But the concentrations of MDA either in the group injected with ADM 6 hrs before trauma or the group injected with ADM 12 hrs after trauma were lower than that in the trauma group（P0.05）.Conclusions：Extraneous ADM can exert a protective effect on renal trauma by controlling the concentration of Ang-Ⅱand MDA.More importantly,ADM injected before trauma shows stronger effect on renal trauma than that injected after trauma.

关 键 词：肾脏 损伤 肾上腺素质素 血管紧张素Ⅱ 丙二醛 

分 类 号：R641[医药卫生—外科学]