机构地区:[1]温州医学院附属第一医院实验诊断中心,温州325000 [2]温州医学院附属第二医院检验科,温州325000 [3]温州市中心血站,,温州325000 [4]温州市体检中心,温州325000 [5]瑞安市人民医院检验科 [6]乐清市人民医院检验科
出 处:《中华检验医学杂志》2011年第2期147-151,共5页Chinese Journal of Laboratory Medicine
摘 要:目的 调查温州地区健康成人RET—He及网织红分群值正常值范围。方法 选取2008年8月至2010年2月温州地区1000名健康体检者,并按性别和年龄分组。按性别分组:男506名,女494名。按年龄分组:20—40岁组341名;〉40~60岁组316名;〉60岁组343名。采集所有调查对象2ml乙二胺四乙酸二钾(EDTA—K:)抗凝静脉血,利用SysmexXE-2100五分类自动血细胞分析仪对1000名健康成人外周血RET—He及网织红分群值(IRF、LFR、MFR、ttFR)进行检测,确定其正常值范围。仪器测定血标本之前进行校准和验证,所有标本采集后2h内完成检测。RET—He、RET绝对值、RET百分数为正态分布数据,采用(牙一1.96s,面+1.96s)确定正常值范围。网织红分群值(IRF、LFR、MFR、HFR)为偏态分布数据,采用百分位数(P2.5,P97.5)来确定正常值范围。结果男性RET—He均值为(33.91±1.77)pg,正常值范围为(30.44—37.38)Pg,女性均值为(33.97±1.85)pg,正常值范围为(30.34—37.60)Pg,差异无统计学意义(t=-0.533,P〉0.05)。男性RET绝对值均值为(0.051±0.023)×10^12/L,正常值范围为(0.006~n096)×10^12/L,女性均值为(0.037±0.026)X10^12/L,正常值范围为(0~0.088)×10^12/L,差异有统计学意义(t=8.409,P〈0.05)。男性RET百分数均值为(1.041±0.459)%,正常值范围为(0.141~1.941)%,女性均值为(0.869-1-0.603)%,正常值范围为(0~2.051)%,差异有统计学意义(t=5.074,P〈0.05)。男性LFR、MFR、HFR、IRF正常值范围分别为0.951(0.866~0.988)、0.046(0.010~0.114)、0.003(0~0.023)、0.050(0.012~0.134),女性分别为0.096(0.880~0.991)、0.039(0.008~0.113)、0.002(0~0.016)、0.040(0.009~0.121),差异均有统计学意义(Z=Objective To investigate the normal ranges of RET-He and reticulocyte grouping parameters of healthy adults in Wenzhou. Methods A total of 1 000 samples from healthy adults were collected in Wenzhou from August 2008 to February 2010. There were 506 males and 494 females. The individuals were stratified based on age into three groups : 20 to 40-year-old group ( n = 341 ) , 〉 40 to 60- year-old group (n =316) , and 〉60-year-old group (n =343). All blood samples were drawn in EDTA-K2 anticoagulated blood. Peripheral RET-He and reticulocyte grouping parameters ( IRF, LFR, MFR, HFR) were determined by Sysmex XE-2100 automated hematology analyzer. The instrument was calibrated and validated before testing. All specimens were analyzed within 2 hours. RET-He, RET absolute value and RET percentage were normal distribution, the normal range was established by using (χ-1.96s, χ + 1.96 s) method. IRF, LFR, MFR and HFR data were skewed distribution, the normal range was established by using ( P2.5 , P97.5 ) percentile method. Results The mean and normal range of RET-He were ( 33.91 ± 1.77 ) pg and (30.44 - 37.38) pg in male, (33.97 ± 1.85) pg and (30.34 - 37.60) pg in female, respectively. There was no significant difference between the two groups ( t = - 0. 533, P 〉 0. 05 ). The mean and normal range of RET absolute value were (0. 051 +0. 023) x 10^12/L and (0. 006 - 0. 096) x 10^12/L in male,which were (0. 037 +0. 026) x 1012/L and (0 -0. 088) x 10^12/L in female, there was significant difference between the two groups ( t = 8.409 ,P 〈 0. 05 ) . The mean and normal range of RET percentage in male were( 1. 041 + 0. 459)% and (0. 141 -1. 941 )% , and in female were (0. 869 ± 0. 603)% and (0 - 2. 051 ) % , which showed significant difference ( t = 5. 074, P 〈 0. 05 ). The normal ranges of LFR, MFR, HFR,IRF in male were 0. 951 (0. 866 -0. 988) ,0. 046(0. 010 -0. 114) ,0. 003(0 -0. 023) ,0. 050(0. 012 -0. 1
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