柴胡疏肝散及其拆方对慢性应激抑郁大鼠模型海马组织ERK1/2 mRNA表达的影响(英文)  

Effect of Chaihu Shugan San and its components on expression of ERK1/2 mRNA in the hippocampus of rats with chronic mild unpredicted stress depression

作　　者：王素娥[1] 胡随瑜[1] 张春虎[1] 邱娟[2] 李云辉[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所国家中医药管理局中医肝藏象重点研究室,长沙410008 [2]中南大学湘雅三医院核医学科,长沙410013

出　　处：《中南大学学报（医学版）》2011年第2期93-100,共8页Journal of Central South University ：Medical Science

基　　金：supported by the National Natural Science Foundation of China(30801506)

摘　　要：目的:探讨柴胡疏肝散及其拆方的抗抑郁作用及机制。方法:将SD大鼠随机分为正常对照组、模型对照组、全方干预组、拆方干预I组和II组、氟西汀对照组。采用慢性轻度不可预见性应激配合孤养复制抑郁模型;运用敞箱试验和蔗糖水消耗实验观察大鼠行为改变;荧光实时定量PCR(FQ-PCR)检测各组大鼠海马组织细胞外调节蛋白激酶(extracellular regulated protein kinases 1/2,ERK1/2)mRNA表达水平。结果:与正常对照组比较,模型组大鼠海马组织ERK1/2 2-ΔΔCT显著降低,差异具有统计学意义(P<0.01),表明模型组海马ERK1/2mRNA表达明显下调。与模型组比较,全方组、氟西汀组大鼠海马组织ERK1/2 2-ΔΔCT均明显升高,差异具有统计学意义(P<0.05或P<0.01);拆方I组ERK1 2-ΔΔCT亦明显上调,差异具有统计学意义(P<0.05);而拆方II组海马组织ERK1/2 2-ΔΔCT均无明显增高,与模型组比较差异无统计学意义(P>0.05)。结论:慢性轻度不可预见性刺激结合孤养可诱导大鼠脑组织ERK1/2mRNA表达下调,柴胡疏肝散可能通过促进海马组织ERK1/2mRNA的表达,改善抑郁大鼠模型的行为学异常,发挥抗抑郁作用;拆方I组也具有较好的抗抑郁作用,但作用不及全方组;拆方II组则无明显抗抑郁作用,但具增强抗抑郁作用。Objective To explore the antidepressant effect and mechanism of Chaihu Shugan San（CHSGS） composition,a compound traditional Chinese herb medicine and its components.Methods Rats were randomly divided into 6 groups： normal control group,model control group,a CHSGS group,a component I group,a component II group and a fluoxetine control group.The depression model was replicated by chronic unpredictable mild stress and single house for 28 d.Behavioral scores of the rats were detected by Open-field test and sucrose solution consumption test,and ERK1/2 mRNA expression in the hippocampus tissue was assayed by real-time fluorescent quantitative PCR.Results ERK1/2 mRNA expression was down-regulated in the depression model group compared with the normal control group（P0.01）.Compared with the model group,ERK1/2 mRNA expression in the CHSGS group and fluoxetine group was both up-regulated（P0.05 or P0.01）;and only ERK1 mRNA expression in the component I group was up-regulated（P0.05）.No significant difference existed between the component II group and the model group（P0.05）.Conclusion Isolated-living condition and chronic mild unpredictable stress can down-regulate the expression of ERK1/2 mRNA in the hippocampus tissue.CHSGS may exert an antidepressant effect through increasing the expression of ERK1/2 mRNA in the hippocampus,component I may play an important role in its antidepression effect,while compatibility of the use of component II can enhance the antidepressant efficacy.

关 键 词：柴胡疏肝散 抑郁症 ERK1/2 实时荧光定量PCR 拆方研究 

分 类 号：R285.5[医药卫生—中药学]