科罗索酸对HepG_2细胞糖消耗以及细胞内糖原的影响  

Effect of Corosolic Acid on Glucose Consumption and Intracellular Glycogen Content in HepG_2 Cell

作　　者：任新凤 陆雪芬 

机构地区：[1]湖州市南浔区人民医院药械科,浙江湖州313009

出　　处：《药学进展》2011年第3期129-132,共4页Progress in Pharmaceutical Sciences

摘　　要：目的:研究糖原磷酸化酶抑制剂科罗索酸对HepG2细胞的葡萄糖消耗以及对正常和应激状态下细胞内糖原含量的影响。方法:不同浓度的科罗索酸与HepG2细胞共同孵育24小时后,用葡萄糖氧化酶法测定单位细胞的糖消耗量,并分别测定正常和应激状态下细胞内糖原的含量。结果:1和10μmol.L-1的科罗索酸能显著增加HepG2细胞的单位细胞糖消耗量,提高HepG2细胞内的糖原含量(P<0.05,P<0.01)。HepG2细胞经去甲肾上腺素处理后,细胞内糖原降解明显增加1,0μmol.L-1的科罗索酸能显著升高细胞内糖原含量水平(P<0.05)。结论:科罗索酸能够增加细胞的葡萄糖消耗,抑制糖原过度降解。Objective：To study the effects of Corosolic acid on glucose consumption and intracellular glycogen content in HepG2 cell line.Methods：Glucose consumption and intracellular glycogen content in HepG2 cells at normal and stress state were respectively determined after the cells were incubated using different concentrations of Corosolic acid for 24 h.Results：Corosolic acid of 1 or 10 μmol ·L-1 could obviously increase cell glucose consumption and intracellular glycogen content.After cells were treated with norepinephrine,glycogen degradation was increased significantly.This trend under stress state could be inhibited by 10 μmol ·L-1 Corosolic acid.Conclusion：Corosolic acid could increase glucose consumption and inhibit glycogen degradation.

关 键 词：科罗索酸 糖原磷酸化酶抑制剂 HEPG2细胞 葡萄糖消耗 糖原 

分 类 号：R965[医药卫生—药理学]