妊娠期高血压和子痫前期孕妇血浆、脐血及胎盘组织神经激肽B的表达及意义  

Expression of neurokinin B in hypertensive disorder complicating pregnancy

作　　者：郭晓霞[1] 张春平[2] 杨年[1] 孙红兵[1] 陈廉[1] 

机构地区：[1]四川省医学科学院四川省人民医院妇产科,成都610072 [2]四川省临床检验中心

出　　处：《中华围产医学杂志》2011年第2期98-101,共4页Chinese Journal of Perinatal Medicine

摘　　要：目的探讨神经激肽B（neurokinin B，NKB）与妊娠期高血压疾病发病的关系。方法采用酶联免疫吸附试验和荧光定量逆转录聚合酶链反应技术检测37例子痫前期患者、50例妊娠期高血压患者、80例正常足月妊娠妇女产前及产后外周血、脐血NKB水平以及NKB mRNA在胎盘组织中的表达，并采用单因素方差分析和配对t检验进行比较，同时与62例正常育龄非孕妇女外周血进行对照。结果（1）正常妊娠组、妊娠期高血压组和子痫前期组产前血浆NKB水平均较正常非孕组显著升高，差异均有统计学意义[（28．2±6．6）μg／L，（31．5±5．2）μg／L，（70．54-8．9）μg／L与（3．2±1．8）μg／L，P〈0．01]。子痫前期组、妊娠期高血压组以及正常妊娠组脐血的NKB水平[（121．4±9．3）μg／L、（60．5±7．2）μg／L和（40．8±6．3）μg／L]均较血浆的水平明显增高，差异有统计学意义（P〈0．01）。（2）妊娠期高血压组产前血浆NKB水平较正常妊娠组有所上升，但差异无统计学意义（P〉0．05）；子痫前期组血浆NKB水平则显著升高，与妊娠期高血压组和正常妊娠组相比差异均有统计学意义（P〈0．01）。与产前血浆NKB水平比较，正常妊娠组、妊娠期高血压组和子痫前期组NKB水平在产后[（14．1±4．2）μg／L，（16．4±3．8）μg／L，（25．4±5．2）μg／L]均显著下降（P〈0．01）。（3）子痫前期组胎盘组织中NKB mRNA的相对表达量较妊娠期高血压组和正常妊娠组明显升高，差异有统计学意义[（3．8±0．6）×10^-3与（1．7±0．4）×10^-3和（1．6±0．3）×10^-3，P〈0．01]。妊娠期高血压组与正常妊娠组比较，胎盘组织NKB mRNA的相对表达量稍有增加，但差异无统计学意义（P〉0．05）。结论异常升高的NKB可能与妊娠期高血压疾病的发生密切相关。Objective To investigate the relationship between the genesis of hypertensive disorder complicating pregnancy and neurokinin B （NKB）. Methods The serum NKB levels of 229 women including non-pregnant women （n = 62）, normal pregnant women （n = 80） and pregnant women with preeclampsia （n= 37） or gestational hypertension （n= 50） were examined by enzyme linked immunosorbent assay. The umbilical blood levels of NKB and the NKB mRNA expression in the pregnant women were also tested by fluorescent quantitative reverse transcription-polymerase chain reaction technique. ANOVA and paired t-test were applied. Results （1）The maternal plasma NKB levels of normal pregnant women, patients with gestational hypertension or preeclampsia were significantly higher than that of non-pregnant women, respectively [（28.2 ± 6.6） μg/L, （31.5 ±5.2） μg/L, （70.5 ±8.9） μg/L vs （3.2 ± 1.8） μg/L, P 〈 0.01]. In preeclampsia, gestational hypertension and normal pregnancy group, the NKB level in umbilical blood [（ 121.4 ± 9.3） μg/L, （60.5±7.2） μg/L, （40.8±6.3） μg/L] were significantly higher than that of maternal plasma（P〈 0.01）. （2） The maternal plasma NKB level in gestational hypertension group was higher than that in normal pregnancy group, while the difference had no statistical significance （P〈0.05）. The maternal plasma NKB level in preeclampsia group was higher than that in gestational hypertension group and normal pregnancy group （P〈0. 01）. Forty-eight hours after delivery, the maternal plasma NKB levels significantly decreased in the normal pregnant, gestational hypertension and preectampsia group[ （14. 1 ± 4. 2） μg/L, （16. 4 ± 3.8） μg/L, （25. 4±5. 2） μg/L], compared to the values before delivery （P〈0.01）. （3） The expression of NKB mRNA in placenta of preeclampsia group was significantly higher than that of gestational hypertension group and normal pregnancy group [（3.8±0.6） ×10^-3 vs （1.7±0.4） ×10^-3 and （1.6±

关 键 词：高血压 妊娠性 神经激肽B 胎血 胎盘 

分 类 号：R714.2[医药卫生—妇产科学]