IGF-1在先天性肥厚性幽门狭窄中的表达  

The expression of IGF-1 in congenital hypertrophic pyloric stenosis

作　　者：张建星[1] 李笃妙[1] 傅冷西[2] 林俊山[1] 宋非无[1] 冯建军[1] 许雅丽[1] 

机构地区：[1]福建医科大学附属第一医院小儿外科,福建福州350005 [2]福建医科大学附属第一医院中心实验室,福建福州350005

出　　处：《中国优生与遗传杂志》2011年第3期32-33,共2页Chinese Journal of Birth Health & Heredity

基　　金：福建省卫生厅青年科研基金项目(2007-2-2)

摘　　要：目的研究细胞因子IGF-1在先天性肥厚性幽门狭窄中的表达。方法应用RT-PCR方法,对16例先天性肥厚性幽门狭窄病例的幽门肌组织及相对应的胃壁肌肉组织的IGF-1mRNA表达水平进行半定量检测。结果幽门肌组织中IGF-1mRNA的水平为0.6858±0.1639,高于胃壁肌肉组织的0.4063±0.0935,差别有统计学意义(P<0.001)。结论先天性肥厚性幽门狭窄患儿幽门肌中IGF-1mRNA表达增加,可能在幽门肌肥厚的过程中起了一定的作用。Objective：To investigate the level of insulin-like growth factor-1 mRNA in congenital hypertrophic pyloric stenosis（CHPS） in children.Methods：This study is based on 16 cases of CHPS that were diagnosised through type-B ultrasonic and surgical intervention.The semi-quantited in 16 cases of pyloric muscle compared to the gastric wall IGF-1 mRNA levels were muscle using reverse transcript polymerase chains reaction（RT-PCR）.Results：The IGF-1 mRNA level was 0.6858±0.1639 in pyloric muscle whileas that was 0.4063±0.0935 in corresponding gastric wall muscle（P0.001）.Conclusions：The up-regulation of IGF-1 expression may partly play a role in the hypertrophy of pyloric muscle in CHPS.

关 键 词：先天性肥厚性幽门狭窄 胰岛素样生长因子-1 逆转录聚合酶链反应 儿童 

分 类 号：R726.5[医药卫生—儿科]