高效液相色谱法测定冬虫夏草口服液中腺苷的含量  

Determination of Adenosine in Cordyceps Sinensis Oral Liquid by High Performance Liquid Chromatography

作　　者：孙倩[1] 张术[2] 储智勇[2] 汪家春[2] 王东[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]海军医学研究所,上海200433

出　　处：《解放军药学学报》2011年第1期72-73,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立冬虫夏草口服液中腺苷含量的测定方法。方法采用高效液相色谱法,色谱柱：Agilent EclipseXDB-C18柱（4.6 mm×150 mm,5μm）,流动相：乙腈-水（7∶93）,检测波长：260 nm,流速：1.0 ml/min,柱温：25℃。结果腺苷在7.5~27.5μg/ml范围内浓度与峰面积呈良好的线性关系,回归方程：Y=12.035X＋41.819,r=0.9999,平均回收率为99.73%,RSD为0.34%（n=9）。结论该方法简单、灵敏、准确,可作为该制剂的含量测定方法。Objective To establish an high performance liquid chromatography method for the determination of adenosine in Cordyceps Sinensis Oral Liquid.Methods Acetonitrile-water（7∶93） was used as the mobile phase with an Agilent Eclipse XDB-C18 column（4.6 mm×150 mm,5 μm） at a flow rate of 1.0 ml/min by high performance liquid chromatography method.The detection wavelength was set at 260 nm under 25℃.Results The calibration of curves was linear within the range of 7.5-27.5 μg/ml for adenosine.The average recovery rate was 99.73% and RSD was 0.34%.The regression equation was：Y=12.035X＋41.819,r=0.9999.Conclusion The method seems simple,sensitive,accurate and can be applied to quality control of the drug.

关 键 词：冬虫夏草口服液 腺苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]