黄芪建中汤对胃黏膜损伤模型大鼠VEGF表达的影响  

EFFECT OF HUANGQI JIANZHONG DECOCTION ON VEGF EXPRESSION OF RATS WITH GASTRIC MUCOSAL LESION

作　　者：汤丽芬[1] 徐升[1] 许祖建[1] 马佳铭[1] 胡建鹏[1] 徐昱[1] 徐国龙[1] 

机构地区：[1]安徽中医学院,合肥230038

出　　处：《中国中医药科技》2011年第2期100-101,128,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：安徽省自然基金资助项目No.070413263X

摘　　要：目的:观察黄芪建中汤对胃黏膜损伤模型大鼠血管内皮生长因子(VEGF)蛋白表达及胃黏膜组织形态学变化的影响。方法:选用健康SD大鼠32只,雌雄各半,随机分为4组:假手术组、模型组、雷尼替丁组和黄芪建中汤组,每组8只。采用改良Okabe法造模,分别观察各组用药1周后的胃组织变化,采用Guth法进行胃黏膜损伤评分;胃黏膜VEGF蛋白表达采用免疫组织化学染色,SABC法测定;HE染色光镜观察病理形态学变化。结果:Guth法胃黏膜损伤评分:模型组评分高于假手术组,各治疗组显著低于模型组,黄芪建中汤组低于雷尼替丁组。VEGF蛋白表达情况:模型组较假手术组减少,黄芪建中汤组与雷尼替丁组明显增加,两组间无显著差异。病理组织学变化:黄芪建中汤组、雷尼替丁组与模型组比较,溃疡有结缔组织增生填充,可见周围黏膜上皮再生。结论:黄芪建中汤可以促进与血管生成相关的VEGF的蛋白表达,改善胃黏膜微循环,发挥胃黏膜损伤修复作用。Objective:To observe the effect of huangqi jianzhong decoction on vascular endothelia growth factor(VEGF) expression of rats with gastric mucosal lesion and the change of morphology Methods:32rats were randomly divided into four groups,sham-operated,model,ranitidine and huangqi jianzhong decoction groups,each group contained 8 rats.Applying Okabe method to make model of gastric mucosal lesion,each group was given the corresponding medicine for 7 days,killing rats to measuring the gastric mucosal lesion by Guth method,VEGF protein expression of gastric mucosal was detected by immunohistochemical method,pathological changes was observed by HE staining.Results:The score of gastric mucosal lesion by Guth method in model group was obviously higher than that of normal group,which was decreased in each treatment group,that was obviously lower in huangqi jianzhong decoction group than that in ranitidine group.The VEGF protein expression of gastric mucosal in model group was less than that in normal group,that was obviously increased in huangqi jianzhong decoction and ranitidine groups.The pathological changes in huangqi jianzhong decoction and ranitidine groups were improved obviously.Conclusion:Huangqi jianzhong decoction can promote the expression of VEGF which is related with angiogenesis,improving microcirculation of gastric mucosal,repairing the gastric mucosal lesion.

关 键 词：胃黏膜损伤/中医药疗法 黄芪建中汤/治疗应用 大鼠 

分 类 号：R285.5[医药卫生—中药学]