异丙酚对新生大鼠空间学习记忆功能及内源性神经干细胞增殖的影响  

The effects of propofol on development of spatial learning and memory and neuron proliferation of neonatal rats

作　　者：王寿平[1] 陈晓彤[2] 苗利萍[1] 张静[1] 彭书崚[1] 

机构地区：[1]中山大学附属第二医院麻醉科,广州510120 [2]中山大学附属第二医院ICU,广州510120

出　　处：《中华行为医学与脑科学杂志》2011年第3期208-210,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金（30772091）;2007年贝朗麻醉科学研究基金;广东省卫生厅基金资助项目（A2009183）

摘　　要：目的探讨不同剂量的异丙酚对新生大鼠空间学习记忆功能发育及对内源性神经干细胞增殖的影响。方法5窝新生SD大鼠按照随机区组化分为对照组（C组）、3种不同剂量异丙酚组（P10组、P50组、P50D组），每组15只。P10、P50组分别单次皮下注射异丙酚10mg／kg、50mg/kg；P50D组先后2次皮下注射异丙酚（50mg／kg）；C组注射等容量的脂肪乳剂。3d后用BrdU法检测海马齿状回神经元的增殖；使用水迷宫检测出生后28d的幼鼠空间学习记忆能力。结果免疫组化的检测中，PIO组齿状回单位面积含Brdu阳性细胞的数量[（1225±154）个／mm^2，P〈0．05]明显多于Con组[（840±76）个／mm^2]，而P50D组[（225±66）个／mm^2，P〈0．05]明显减少。水迷宫试验中，P50D组空间探索的潜伏期[（42．68±6．18）S，P〈0．05]明显长于Con组[（15．12±3．43）s]，P50D组目标象限停留时间[（32．18±5．38）s，P〈0．05]明显少于Con组[（55．66±8．57）S]，而P50组、P10组与Con组的差异均无统计学意义。结论大剂量异丙酚可以抑制新生大鼠的空间学习、记忆能力发育，并可抑制内源性神经干细胞增殖；小剂量的异丙酚反而可以促进内源性神经干细胞增殖。Objective To investigate the effects of propofol on the development of spatial learning and memory and neuron proliferation of neonatal rats at different doses. Methods 60 neonatal rats were divided into four groups among per litter by using a randomized block design. Three different doses of propofol group were induced with propofol 10 mg/kg （ group P10 ）, 50 mg/kg （ group P50 ） or 50 mg/kg twice （ group P50D） by subcutaneous injection respectively. Neuron proliferation at dentate gyrus was detected by using BrdU marker 3 days later. Morris water maze test was carried out on postnatal day 28. Escape latency, time 5n probe quadrant were recorded. Results Compared to the control group, neuron marked with BrdU at dentate gyrus in group P50D was significantly decreased （ （ 840± 76 ） vs （ 225 ± 66 ）, P 〈 0.05 ） , group P10 was significantly increased （ （ 840 ± 76 ） vs （ 1225 ± 154）, P 〈 0.05 ）. Compared to the control group,latency of group PSOD was significantly increased （ （ 15.12 ± 3.43）s vs （42.68 ± 6. 18 ）s, P 〈 0.05 ）, time in probe quadrant of group PSOD were significantly decreased （ （55.66 ±8.57）s vs （32.18 ±5.38）s, P〈0.05）. Compared to the control group,there was no significant difference between group P50 and group PIO. Conclusion Propofol given to seven-day-old rats with 50 mg/kg twice by subcutaneous injection suppresses neuron proliferation and impairs development of memory and learning in neonatal rats,but propofol given with 10 mg/kg once promotes neuron proliferation.

关 键 词：异丙酚 新生 增殖 学习记忆 脑发育 

分 类 号：R965[医药卫生—药理学]