壳聚糖-海藻酸钙微球荧光标记反应对微球膜强度影响的研究  

Study on the Impact of Fluorescence Labeling Reaction of Chitosan-calcium Alginate Microsphere on Microsphere Membrane Strength

作　　者：范妮[1] 薛伟明[2] 

机构地区：[1]陕西教育学院化学与化工系,陕西西安710100 [2]西北大学化工学院,陕西西安710069

出　　处：《四川师范大学学报（自然科学版）》2011年第2期231-235,共5页Journal of Sichuan Normal University（Natural Science）

基　　金：国家自然科学基金(50373046)资助项目

摘　　要：以壳聚糖-海藻酸钙载药微球给药后的体内检测为研究背景,探讨不同反应条件下壳聚糖-海藻酸钙微球荧光标记反应对微球膜在模拟体液中的强度影响.以壳聚糖、海藻酸钠为微球制备载体材料,以异硫氰酸酯(FITC)为荧光标记物对微球进行荧光标记.采用标记了FITC的微球在模拟体液中的膨胀率来表征微球膜的相对强度.采用相对分子质量为50 000、脱乙酰度85%、荧光标记浓度0.01 g/mL的壳聚糖,成膜液浓度0.015 g/mL,成膜时间30 min制备出的荧光微球在模拟胃、肠液中表现出良好的膜强度及稳定性.为研究壳聚糖-海藻酸钙载药微球在体内的分布、吸收、降解特性提供了适宜的入体实验条件.This paper investigates the impact of fluorescence labeling reaction of chitosan-calcium alginate microsphere for labeling on microphere membrane strength in simulated body liquid under different conditions in order to detect such microspheres in vivo.With chitosan and sodium alginate as materials for preparing microsphere and with isothiocyanate FITC as fluorescence label for labeling microspheres,the rate of expansion of FITC-labeled chitosan-alginate microspheres is determined to characterize microsphere membrane strength.The FITC-labeled microspheres are prepared with the FITC-labeled chitosan of which concentration is 0.015 g/mL reacting 30 min with calcium alginate microspheres.On the other hand,chitosan molecular weight is 50 000,the degree of deacytylation is 85% and the solution concentration is 0.01 g/mL.Such FITC-labeled microspheres show better membrane strength and stability.The appropriate experimental conditions are provided to study the distribution、absorbency and biodegradation characteristics of drug-loaded chitosan-alginate microsphere in vivo.

关 键 词：壳聚糖 海藻酸钙 微球 荧光标记 膜强度 

分 类 号：TQ460.6[化学工程—制药化工]