不对称二甲基精氨酸对THP-1巨噬细胞源性泡沫细胞内胆固醇含量的影响  

Effects of ADMA on cholesterol content in THP-1 macrophage-derived foam arils

作　　者：高小花[1,2] 王庸晋[2,3] 王金胜[1] 王治平[3] 李启富[3] 

机构地区：[1]山西医科大学附属第一临床医院心内科,山西省030001 [2]长治医学院心血管研究所 [3]长治医学院附属和平医院心血管病内科

出　　处：《中国心血管病研究》2011年第2期144-147,共4页Chinese Journal of Cardiovascular Research

基　　金：国家自然科学基金（30840043）;山西省高校科技研究项目（20081033）

摘　　要：目的 观察不对称二甲基精氨酸（ADMA）对THP-1巨噬细胞源性泡沫细胞内胆固醇含量的影响.方法 THP-1单核细胞给予160nmol/L佛波酯（PMA）孵育48h,诱导分化成巨噬细胞,与80mg/L氧化型低密度脂蛋白（ox-LDL）孵育24h,使巨噬细胞转化为泡沫细胞,用油红O染色在光镜下鉴定泡沫细胞形态及变化.再以不同浓度ADMA（1μmol/L、5μmol/L、10μmol/L、20μmol/L、30μmol/L）作用泡沫细胞24h,以20μmol/L ADMA作用泡沫细胞不同时间（0h,6h,12h,24h,48h）,酶比色法检测泡沫细胞内胆固醇的含量.结果 ①单核细胞加人PMA诱导48h后,分化为巨噬细胞,再经ox-LDL诱导24h后转化为泡沫细胞.②与对照组相比,5μmol/L、10μmol/L、20μmol/L,30μmol/L的ADMA作用于,THP-1巨噬细胞源性泡沫细胞24h后,细胞内胆固醇含量均明显增加（P＜0.01）,③与对照组相比,20μmol/L的ADMA作用于THP-1巨噬细胞源性泡沫细胞12h,24h,48h,细胞内胆固醇含量均明显增加（P＜0.01）.结论 ①单核细胞株THP-1经PMA诱导后,可分化为巨噬细胞,再经ox-LDL诱导后,可转化为泡沫细胞.②ADMA可以增加THP-1巨噬细胞源性泡沫细胞内胆固醇的含量.Objective To investigate the effects of Asymmetric dimethylarginine （ADMA） on cholesterol content in THP-1 macrophage-derived foam cells. Methods Cultured THP-1 monocyte cell was induced into macrophages by 160 nmol/L phorbol myristate acetate （PMA） for 48 h and then incubated with 80 mg/L oxidized low density lipoprotein （α-LDL） for 24 h,macrophages were induced into foam cells. Transmission light microscope （using red oil O staining technique） was applied to appraise the morphology and change of foam cells. After ADMA of different concentrations（ 1μmol/L, 5 μmol/L, 10μmol/L, 20 μmol/L, 30 I.Lmol/L） were incubated with THP-1 macrophage-derived foam cells for 24 h and 20 ~mol/L ADMA was incubated with THP-1 macrophage- derived foam cells for 0 h,6 h, 12 h,24 h,48 h, the cholesterol content of foam ceils was measured by enzyme assay. Results （1）The mononuclear cells line THP-1 could be differentiated into maerophages after 48 h induction by the PMA and into foam ceils after 24 h induction by ox-LDL. （2）Compared with the control group, treatment of THP-1 macrophage-derived foam ceils with 5 μmol/L, 10 μmol/L,20 μmol/L,30μmol/L ADMA for 24 h resulted in an increase in the cholesterol content of foam cells （P〈0.01）. （3）Compared with the control group, treatment of THP-1 macrophage-derived foam cells with 20 μmol/L ADMA for 12 h,24 h,48 h, resulted in an increase in the cholesterol content of foam cells （P〈0.01）. Conclusion （1）mononuclear cells line THP-1 could differentiate into macrophage after induction by PMA and into foam cells after induction by ox-LDL. （2）ADMA could increase the cholesterol content of foam cells.

关 键 词：巨噬细胞 泡沫细胞 不对称二甲基精氨酸 胆固醇 

分 类 号：Q95-33[生物学—动物学] R541.4[医药卫生—心血管疾病]