氧化型高密度脂蛋白对内皮细胞的损伤作用  

The injury of Oxidized HDL on endothelial cell

作　　者：彭湘萍[1] 

机构地区：[1]吉首大学医学院生理系,湖南省416000

出　　处：《中国心血管病研究》2011年第3期228-230,共3页Chinese Journal of Cardiovascular Research

基　　金：吉首大学校级科研项目资助（编号：09JD019）

摘　　要：目的 探讨氧化高密度脂蛋白（ox-HDL）对人脐静脉内皮细胞（HUVECs）的损伤作用.方法 体外培养HUVECs与不同浓度（50～200 mg/L）ox-HDL共孵育,用普通光镜和荧光显微镜分别观察HUVECs细胞形态及核形态的变化,用噻唑兰（MTT）比色法检测细胞存活状况.结果 ox-HDL使内皮细胞数目减少,形态改变;Hoechst 33258染色后可见大量的细胞核浓缩呈高亮度蓝色荧光的凋亡细胞.MTT结果显示,细胞存活率下降,且呈剂量依赖性.结论 ox-HDL可导致内皮细胞受损,从而促进动脉粥样硬化的发生、发展.Objective To explore the effect of ox-HDL on human umbilical vein endothelial cell. Methods HUVECs were incubated with ox-HDL（50,100,200 mg/L） for 24 hours. The change of cell shape and nuclear morphology were observed by light microscope and nuclear staining with lqoechst 33258. The cells survival percentage was detected by MTT assay. Results Compared with the control group, the number of HUVECs cells incubated with ox-HDL decreased and the shape changed. Cells appeared typical characteristics of apoptosis, including apoptotic nuclear condensation. The survival rate of cells was reduced by MTT assay. Conclusion Ox- HDL can damage HUVECs.

关 键 词：氧化型高密度脂蛋白 血管内皮细胞 损伤 

分 类 号：Q95-33[生物学—动物学] R541.4[医药卫生—心血管疾病]