卡介苗对哮喘小鼠肺组织中PPAR-γ表达的影响及意义  

Effect of Bacillus Calmette-Guerin on Expression of PPAR-γ in Lung Tissue of Asthmatic Mice and Its Significance

作　　者：曾晓媛[1] 黄信刚[1] 陈红梅[1] 陈健[2] 

机构地区：[1]中南大学湘雅二医院呼吸科,湖南长沙410011 [2]长沙市中心医院胸7科,湖南长沙410011

出　　处：《现代生物医学进展》2011年第3期409-412,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨卡介苗对哮喘小鼠肺组织中PPAR-γ表达的影响及其意义。方法:30只健康雄性昆明种小鼠随机化原则分成正常对照组(A组)、哮喘模型组(B组)和卡介苗干预组(C组),每组10只。卵蛋白致敏法复制哮喘模型,B组小鼠于实验的第1、8、15天分别给予卵蛋白与氢氧化铝混合腹腔注射。第22天开始给予卵蛋白溶液以压缩雾化器为动力雾化吸入激发哮喘,每日一次,每次30min。连续激发7天;C组小鼠每周一次皮内注射0.025mgBCG,连续3次,距首次皮内注射4周后按B组方法致敏和激发;A组予以等量生理盐水代替致敏液及雾化液进行腹腔注射与雾化吸入。检测细支气管炎症细胞浸润及肺组织病理,RT-PCR和western blotting方法检测肺组织PPAR-γ的表达情况。结果:哮喘组出现了明显气道炎症细胞浸润,上皮脱落、炎性细胞渗出、结构紊乱、PPAR-γ表达下降。卡介苗干预组炎症细胞数下降,炎症反应减轻,PPAR-γ表达明显增加,差异具有统计学意义(P<0.01)。结论:卡介苗可通过上调表达PPAR-γ,减少炎症细胞的浸润,抑制炎症反应,从而可能防止气道重塑,该研究为卡介苗提供新的临床应用领域。Objective：To investigate the effect of bacillus Calmette-Guerin（BCG） on the expression of peroxisome prolifera-tor-activated receptor-γ（PPAR-γ） in the lung tissue of asthmatic mice and its significance.Methods：Thirty adult male Kunming mice were randomly divided into three groups（n=10）：the control group（group A）,asthmatic group（group B）,BCG group（group C）.The asthmatic model was established by the ovalbumin challenge method.Mice in group B were given ovalbumin and aluminum hydroxide mixture by intraperitoneal injection at 1,8,15 day.Mice were inhaled ovalbumin solution drived by compressed Atomizer for asthma once a day（every 30min） from 22 day and continuously challenged for 7 days.Mice in group C were weekly given 0.025mg BCG by in-tradermal injection and continuously injected for 3 times.After 4 weeks,mice were sensitized and challenged by the method as group B.Mice in group A were given normal saline solution.The bronchiole inflammatory cell infiltration,remodeling index and lung tissue histopathological were observed.Expressions of PPAR-γ in lung tissue were assayed by RT-PCR and western blotting.Results：There were obviously bronchiole remodeling response and inflammatory cell infiltration in group B,which can be inhibited by BCG.RT-PCR and western blotting showed that the expression of PPAR-γ in lung tissue was down-regulated in group B compared to that of group A（P0.05）.While the expression of PPAR-γ in lung tissue was increased in group C after BCG treatment.Conclusion：BCG reduced infil-tration of inflammatory cells and inhibited the inflammatory response to prevent airway remodeling by up-regulating the expression of PPAR-γ.The study provides a new clinical application for BCG.

关 键 词：支气管哮喘 过氧化物酶体增殖物激活受体-Γ 卡介苗 肺组织 

分 类 号：Q95-3[生物学—动物学] R562.25[医药卫生—呼吸系统]