不同染色技术和双色荧光原位杂交技术对宽翅菘蓝(Isatis violascens Bunge)的细胞遗传学分析  

Cytogenetic Analysis of Isatis Violascens Bunge by Different Staining Techniques and D-Fluorescent in Situ Hybridization

作　　者：戚大石[1] 汤佳立[2] 

机构地区：[1]徐州医学院,江苏徐州221121 [2]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《现代生物医学进展》2011年第3期485-488,共4页Progress in Modern Biomedicine

摘　　要：为了解十字花科菘蓝属植物宽翅菘蓝(宽翅菘蓝,Isatis violascens Bunge)的染色体结构,本文利用45SrDNA和5SrDNA双色荧光原位杂交、银染技术和CMA3对宽翅菘蓝进行分子细胞遗传学研究。45SrDNA和5SrDNA荧光原位杂交结果显示宽翅菘蓝染色体上有1对45SrDNA信号和1对5SrDNA信号;银染结果显示其中期染色体有1对银染点;CMA3染色结果发现宽翅菘蓝中期染色体存在1对CMA3信号。Objective In order to understand the chromosome structure of I.violascens Bunge,45S rDNA and 5S rDNA dual-flu-orescence in situ hybridization（45S rDNA and 5S rDNA D-FISH）,silver staining and CAM3 staining were used to study the chromo-somes of I.violascens Bunge.In the silver stained metaphase chromosomes of I.violascens Bunge,there were two positive regions;fluo-rochrome Chromomycin A3（CMA） staining showed two positive regions;D-FISH with 45S rDNA and 5S rDNA probes revealed one 18S-5.8S-26S rDNA loci and one 5S rDNA loci with different intensity.

关 键 词：宽翅菘蓝 银染 45SrDNA 双色荧光原位杂交 CMA3染色 

分 类 号：Q343[生物学—遗传学] Q943