红霉素对慢性阻塞性肺疾病大鼠ICAM-1、MMP-9的表达影响  

Effect of Erythromycin on Expressions of ICAM-1 and MMP-9 in Rats with COPD

作　　者：黄玲媚[1] 罗百灵[1] 陈红梅[1] 

机构地区：[1]中南大学湘雅医院呼吸内科,湖南长沙410008

出　　处：《现代生物医学进展》2011年第3期497-501,共5页Progress in Modern Biomedicine

摘　　要：目的:观察慢性阻塞性肺疾病(COPD)大鼠肺组织中ICAM-1及MMP-9的表达及红霉素的干预作用。方法:复制COPD大鼠模型,并用红霉素干预,收集支气管肺泡灌洗液行细胞学计数和分类检查;采用HE染色观察病理形态变化;免疫组化法检测大鼠支气管肺组织ICAM-1、MMP-9的表达。结果:与模型组比较,干预组支气管肺组织中ICAM-1、MMP-9表达显著降低;模型组中ICAM-1、MMP-9的表达与BALF中白细胞总数及中性粒细胞数成正相关;ICAM-1与MMP-9的表达成正相关。结论:COPD大鼠肺组织中的ICAM-1、MMP-9表达明显升高,可能与COPD的发病机制有关;红霉素可降低ICAM-1、MMP-9的表达,可能是红霉素在COPD中抗炎症反应的作用机制之一。Objective：To explore the role of ICAM-1 and MMP-9 in the pathogenesis of COPD,and to study the effects of ery-thromycin（EM）on lung ICAM-1 and MMP-9 expression in vivo experiment of COPD rats.Methods：We established COPD rat models and use Erythromycin to interven.BALF were collected to count the total and differential cell counts.The pathological changes were observed.The expressions of ICAM-1 and MMP-9 were detected by immunohistochemistry.Results：Compared to the COPD group,the expression of ICAM-1 and MMP-9 in control group were significantly dcreased;Correlations analyze in COPD group：Both positive cor-relations were demonstrated in the expression of ICAM-1 and MMP-9 with WBC counts,PMN counts,PMN counts;Positive correla-tions were demonstrated between the expression of ICAM-1 and MMP-9;Conclusions：The difference of expression of ICAM-1 and MMP-9 between COPD group and control group have statistical significance,which indicate that ICAM-1 and MMP-9 may play impor-tant role in the formation of COPD;EM may play a certain protective role in the treatment of COPD through decreasing the expression of ICAM-1 and MMP-9 and weakening the inflammatory response.

关 键 词：红霉素 慢性阻塞性肺疾病 ICAM-1 MMP-9 

分 类 号：Q95-3[生物学—动物学] R563[医药卫生—呼吸系统]