白毛藤总苷诱导人食管癌细胞Ec-9706凋亡及其作用机制的研究  

STUDY OF SOLAMUM LYRATUM THUNB TOTAL GLYCOSIDES LNDUCING APOP-TOSIS OF HUMAN ESOPHAGEAL EPITHELIA EC-9706 CELLA AND LTS MECHANISM

作　　者：杨旭东[1] 包海花[1] 董凯[1] 

机构地区：[1]黑龙江省牡丹江医学院,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2011年第1期7-9,共3页Journal of Mudanjiang Medical University

基　　金：黑龙江省教育厅科学技术研究项目(11531398)

摘　　要：目的:研究白毛藤总苷诱导人食管癌细胞凋亡的作用及其机制。方法:荧光显微镜观察不同浓度白毛藤总苷诱导Ec-9706细胞凋亡的作用,RT-PCR检测Bcl-xl、p53基因表达量的变化。结果:白毛藤总苷具有诱导Ec-9706细胞凋亡的作用,并且上调p53基因、降低Bcl-xl基因的表达。结论:白毛藤总苷促进Ec-9706细胞凋亡,其机制可能与激活p53基因、抑制Bcl-xl基因表达有关。Objective：To study the effect of Solamum lyratum Thunb total glycosides on apoptosis of Human esophageal epithelia Ec-9706 cells and its mechanism.Methods：Ec-9706 cells were treated with various doses of Solamum lyratum thunb total glycosides,We observed apoptosis of Ec-9706 cells by fluorescence microscope.We detected the levels of Bcl-xl and p53 gene expression in all groups by RT-PCR.Results：Solamum lyratum thunb total glycosides has obviously apoptosis-inducing effects on Ec-9706 cells and could effectively decrease the level of Bcl-xl gene expression and improve the level of p53 gene expression.Conclusion：Solamum lyratum thunb total glycosides could induce apoptosis of Ec-9706 cells probably through regulating Bcl-xl gene expression down and regulating p53 gene expression up.

关 键 词：BCL-XL基因 P53基因 细胞凋亡 白毛藤 

分 类 号：R34[医药卫生—基础医学]