甜菜单体附加系M14的RNA提取方法比较  

Comparison of RNA Extracting Methods of M14,Themonosomic Addition Lines of Beta vulgaris

作　　者：张先成[1] 赵晓宇[1] 孟利强[1] 王立谦[2] 

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]黑龙江省科学院,黑龙江哈尔滨150001

出　　处：《生物技术》2011年第1期45-47,共3页Biotechnology

摘　　要：目的:对甜菜无融合生殖单体附加系M14品系的叶和花总RNA提取方法进行对比,消除其提取产量低、RNA降解、DNA干扰、多糖干扰、蛋白质干扰和杂质干扰等缺陷。方法:采用6种方法进行RNA提取分析。结果:在RNA提取过程中加入约1/3倍体积柠檬酸钠等除糖剂,能够有效去除多糖干扰,大大提高所提RNA的质量。获得最佳RNA提取方法。Objective：To Eliminate the defects such as low yield of its extraction,RNA degradation,DNA interference,polysaccharide interference,protein interference and impurity,in the process of the RNA extraction of the leaf and flower of M14,Themonosomic Addition Lines of Beta vulgaris.Method：Six RNA extraction methods were Comparative analyzed.Result：Adding 1/3 times the volume of sodium citrate during the process of RNA extraction can effectively remove the interference of polysaccharides,enhance the quality of the proposed RNA greatly.So,the best RNA extracting method was got.

关 键 词：甜菜 无融合生殖 单体附加系 RNA提取 

分 类 号：Q78[生物学—分子生物学]