正畸力作用下兔牙周组织中PI3K的表达  

Expression of PI3K in Rabbit Periodontal Tissues Remodeling during Orthodontic Tooth Movement

作　　者：刘奕[1] 孙素芬[1] 

机构地区：[1]中国医科大学口腔医学院奉天门诊.辽宁省口腔医学研究所,辽宁沈阳110013

出　　处：《口腔医学研究》2011年第2期141-144,共4页Journal of Oral Science Research

基　　金：辽宁省科学技术计划项目(编号:2009225010-27);沈阳市科学技术计划(编号:1072033-1-00)

摘　　要：目的:探讨正畸力作用下兔牙周组织中PI3K在正畸牙移动牙周组织改建过程中的作用。方法:选用24只日本大耳白兔建立正畸牙移动的动物模型,将实验动物上颌右侧戴矫治器,作为实验组;左侧未戴矫治器,作为对照组。分别在戴矫治器后3、5、7、14d各处死6只实验动物。用实时荧光定量PCR及Western blot免疫印迹分析方法对PI3K表达的变化进行检测。结果:实时荧光定量PCR结果显示,加力3d后牙周组织中PI3KmRNA表达增强,7d后牙周组织中PI3KmRNA表达明显增强,随后缓慢下降,与对照侧相比,其差异有统计学意义(P<0.01)。Western blot免疫印迹分析与RQ-PCR结果一致。结论:在正畸牙移动过程中,兔牙周组织中PI3K的表达明显增强,提示PI3K参与牙周组织改建,并在牙周组织改建中起重要作用。Objective：To study the role of PI3K in periodontal tissues remodeling during orthodontic tooth movement.Methods：Twenty-four rabbits were chosen to establish rabbit models for the study.The right maxillary teeth of each animal treated by orthodontics were the test side, and the untreated left teeth were set the control side.The animals were sacrificed after 3,5,7,14 d,respectively.The prepared tissue specimens were processed to study the expression of PI3K in periodontal tissues by real-time quantitative PCR and Western blot techniques.Results：Real-time quantitative PCR showed that the expression of PI3K mRNA dramatically changed after 3 d.The expression of PI3K mRNA in treating periodontal tissues was higher than that in the control side,especially after 7 d,and then decreased.Compared with the control side,there was significant difference（P〈0.01）.The expression of Western blot was the same as the real-time quantitative PCR.Conclusion：Higher expression of PI3K during orthodontic tooth movement plays an important role in the process of periodontium remodeling.

关 键 词：正畸力 PI3K 实时荧光定量PCR 牙周组织改建 

分 类 号：R783.5[医药卫生—口腔医学]