杭白菊对衰老小鼠肝组织SOD、GSH-PX和MDA表达的影响  

Effects of Chrysanthemum morifolium on expression of SOD,GSH-PH and MDA of liver tissue in D-gal-induced aging mice model

作　　者：于亚威 徐广涛[2] 潘晓燕[2] 周新妹[2] 沈岳良[3] 

机构地区：[1]嘉兴市第二医院病理科,浙江嘉兴314001 [2]嘉兴学院医学院病理学教研室,浙江嘉兴314001 [3]浙江大学医学院生理学教研室,浙江杭州310058

出　　处：《温州医学院学报》2011年第1期53-55,共3页Journal of Wenzhou Medical College

基　　金：浙江省科技计划资助项目(G20020578);嘉兴市科技计划资助项目(01129)

摘　　要：目的:研究杭白菊对衰老模型小鼠肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)表达的影响,探讨其对肝组织的保护作用。方法:以D-半乳糖建立人工致衰老小鼠并给予杭白菊灌胃处理,ICR雌性小鼠64只,每组16只,随机分成4组,空白对照组、D-半乳糖对照组、维生素E处理组和杭白菊处理组,检测小鼠血清和肝组织中SOD、GSH-PX活性及MDA的含量。结果:杭白菊处理组血清、肝中SOD、GSH-PX含量均高于D-半乳糖对照组(P<0.01),血清MDA低于D-半乳糖对照组(P<0.01)。杭白菊处理组血清、肝中SOD、GSH-PX、血清MDA含量与维生素E处理组及空白对照组差异无统计学意义(P>0.05)。结论:杭白菊主要通过提高抗氧化酶活性,清除或减少氧自由基和脂质过氧化物而实现其保护肝组织的作用。Objective： To investigate the protective effects of dendranthema morifolium（DM）on liver tissue and expression of SOD,GSH-PX and MDA of liver tissue in D-gal-induced aging mice model.Methods： Sixty-four ICR female mice were randomly divided into control group,D-galactose control group,Vit-E treatment group and the dendranthema morifolium（DM）treatment group,16 rats in each.The aging mice model was established by D-galactose and dealt with DM to measure the content of SOD,GSH-PX and MDA in liver and serum.Results： The content of SOD,GSH-PX in serum and liver in DM group was higher than D-gal-induced aging mice model group（P0.01）,but MDA was lower（P0.01）.The content of SOD,GSH-PX,MDA in serum and liver in DM group was not significantly difference than that in Vit-E group and normal group（P0.05）.Conclusion： The protective effects of DM on liver tissue in D-gal-induced aging mice model are due to enhance SOD and GSH-PX,reduce MDA.

关 键 词：杭白菊 D-半乳糖 肝脏 小鼠 衰老 

分 类 号：R339.3[医药卫生—人体生理学]