西瓜根际细菌JK-3的鉴定及其拮抗性的初步研究  

Preliminary study on identification of watermelon rhizosphere bacterium JK-3 and its antagonism

作　　者：陈文俊[1,2] 曾蓉[1] 陆金萍[1] 宋荣浩[1] 童蕴慧[2] 戴富明[1] 

机构地区：[1]上海市农业科学院植物保护研究所上海市园艺设施重点实验室,上海201106 [2]扬州大学植物保护系,扬州225009

出　　处：《上海农业学报》2011年第1期68-72,共5页Acta Agriculturae Shanghai

基　　金：上海市科委重大项目(08DZ1900400和08DZ1906600)资助

摘　　要：JK-3菌株是从西瓜根际筛选得到的一种革兰氏阳性菌,大小为3.0～4.2μm×0.7～1.0μm,菌落呈淡黄色、圆形、隆起、无褶皱、边缘整齐。扫描电镜观察表明,该菌周生鞭毛,卵圆形芽孢,孢囊膨大。培养4d后菌落表面有较多粘性物质生成。通过16S rDNA测序、常规生理生化方法以及Biolog微生物自动鉴定系统的碳源测定等3种方法鉴定出该根际细菌为多粘类芽孢杆菌。平板对峙培养和孢子萌发试验表明,该菌株对多种植物病原真菌具有良好的拮抗作用并能产生抗生物质。The strain JK-3 screened from watermelon roots is a kind of Gram-positive bacterium with rod-shape（3.0～4.2μm×0.7～1.0μm）,and the colony assumes light yellow and an appearance of round,bulge,no wrinkle and regular edge.Under a scanning electron microscope the bacterium is peritrichous and has an oval spore and a swelling cyst.Cultured for 4 days,the colony produces much sticky substance on its surface.The JK-3 is identified as Paenibacillus polymyxa by three methods-16S rDNA sequencing,conventional physiological and biochemical analysis and the Biolog automated microbes identification system.The dual culture and spore germination tests showed that the JK-3 has high antagonism to many important phytopathogens and can produce antibiotic substance.

关 键 词：根际细菌 鉴定 拮抗性 多粘类芽孢杆菌 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]