甘草黄酮对大强度耐力运动大鼠肾脏组织细胞凋亡及Bax、Bcl-2蛋白表达的影响  

Effect of High-intensity Endurance Exercise on Apoptosis and Bax,Bcl-2 Protein Expression with Glycyrrhiza Flavonoids in Rat Nephridial Tissue

作　　者：陈艳艳[1] 

机构地区：[1]西北大学体育部,陕西西安710069

出　　处：《北京体育大学学报》2011年第2期61-64,共4页Journal of Beijing Sport University

基　　金：陕西省自然科学基金资助项目(SJ08F22)

摘　　要：目的:探讨甘草黄酮对大强度耐力运动大鼠肾脏组织细胞凋亡及Bax(促凋亡基因,B apoptosis cell matrixgene)、Bcl-2(B细胞淋巴瘤基因-2,B cell lymphoma gene-2)蛋白表达的影响。方法选取SD雄性健康大鼠24只,随机分为安静组、大强度运动组和运动加药组;采用跑台训练6周后取材,应用免疫组织化学法测检测各组大鼠肾脏组织细胞凋亡及Bax和Bcl-2蛋白表达的变化。结果:与安静对照组相比,大强度耐力运动组和运动加药组大鼠细胞凋亡指数AI均有不同程度增加;大强度运动组Bax、Bcl-2蛋白表达(MOD)均呈非常显著差异性(P<0.01),运动加药组Bax蛋白表达(MOD)均呈非常显著差异性(P<0.01);运动加药组Bcl-2蛋白表达(MOD)与大强度运动组具有非常显著差异性(P<0.01);大强度运动组和运动加药组Bax/Bcl-2比值分布呈不同程度的显著差异性(P<0.05,P<0.01)。结论:甘草黄酮能够提高大强度运动大鼠抗氧化能力,降低肾脏组织自由基的产生,抑制肾脏组织细胞的凋亡。Objective： To explore glycyrrhiza flavonoids on the rat nephridial tissue of cell apoptosis and Bax,Bcl-2 protein expression with high-intensity endurance exercise.Methods： The twenty-four healthy male rats were randomly divided into control group,high-intensity exercise group and exercise plus glycyrrhiza flavonoids group.After 6 weeks of treadmill training,by using the box of reagent and immunity histochemistry,the research examined the change of cell apoptosis and Bax,Bcl-2 protein expression on each group.Results： Compared with the quiet groups,high-intensity endurance exercise group and exercise dosing rats AI apoptosis index increased in varying degrees;High-intensity exercise group （MOD） were significant different（P0.01）,exercise plus drug group Bac protein expression（MOD）were significant different（P0.01）;Exercise plus drug group Bcl-2 protein expression（MOD） with the high-intensity exercise group had significant difference（P0.01）,high-intensity exercise group and exercise plus drug group Bax/Bcl-2 ratio of distribution has significantly different degrees of difference（P0.05,P0.01）.Conclusion：glycyrrhiza flavonoids can improve the antioxidant capacity rats in high intensity exercise,reduce renal tissue of free radicals and inhibit kidney tissue cell apoptosis.

关 键 词：甘草黄酮 大强度耐力训练 肾脏 细胞凋亡 BAX Bcl-2 

分 类 号：G804.7[文化科学—运动人体科学]