毛细管电泳法测定多种水溶性金属离子  

Water-solubility metal ions detection for complex samples using a capillary electrophoresis

作　　者：石美[1] 高庆宇[1] 陆永超[1] 冯加民[1] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221116

出　　处：《中国矿业大学学报》2011年第2期305-309,共5页Journal of China University of Mining & Technology

基　　金：国家自然科学基金项目(20943005);江苏省自然科学基金项目(BK2007037);中国矿业大学青年基金项目(OH060104)

摘　　要：开发了一种将毛细管电泳用于含水溶性金属离子样品分离检测的间接紫外法.采用咪唑为紫外生色剂,用醋酸调节pH值,考察了咪唑和醋酸浓度(pH值)对分离效果的影响,并对分离电压和检测波长作了系统优化.结果表明:当咪唑浓度为15mmol/L(pH=3.7),分离电压为18kV时,可在8.5min内实现K+,Ba2+,Ca2+,Na+,Mg2+,Mn2+,Zn2+,Cd2+,Li+和Cu2+10种金属离子的基线分离,各金属离子的检测限、线性范围和标准工作曲线相关系数分别为0.01～0.21mg/L,0.06～60mg/L和0.999 5～0.999 8之间,标准样品回收率为96.4%～102.6%,5次测定的相对标准偏差在0.8%～4.5%之间.通过对11个样品的检测分析表明,该法可以用于这些金属离子在复杂基质中的常规检测.An indirect UV method performed by a capillary electrophoresis was developed to separate water-solubility metal ions.The background electrolyte merely contains 15 mmol/L chromophore imidazole and pH adjustment acetic acid（pH=3.7）,which also functioned as a complexing reagent.The effects of imidazole and concentration of acetic acid on separation efficiency were discussed.The separation voltage and detection wavelength were optimized.The results show that all cations,including K＋,Ba2＋,Ca2＋,Na＋,Mg2＋,Mn2＋,Zn2＋,Cd2＋,Li＋and Cu2＋,can be completely separated within 8.5 min under hydrodynamic mode with a running voltage of 18 kV at 25 oC and pH=3.7.The limits of detection of 0.01~0.21 mg/L,linear ranges of 0.06~60 mg/L,relative coefficients of calibration curves of 0.999 5~0.999 8,standard recoveries of 96.4%~102.6% and relative standard derivations of 0.8%~4.5% were achieved,respectively.The analysis results obtained from eleven real samples demonstrate that the developed method is convenient for routine analysis in complex samples.

关 键 词：毛细管电泳 间接紫外检测法 咪唑 金属离子 

分 类 号：O657[理学—分析化学]