荷斯坦牛Leptin基因外显子2多态性与繁殖力性状的相关性研究  

Study on Polymorphism in the Exon 2 of Leptin Gene and Its Relationship with Fertility Traits of Holstein Cows

作　　者：李鹏[1,2] 何举 赵国丽[2] 宫艳斌[2] 韩元[2] 王丽 史远刚[1,3,2] 

机构地区：[1]宁夏正鑫牧业科技发展有限公司,银川750002 [2]宁夏大学农学院,银川750021 [3]宁夏四正生物工程技术研究中心,银川750200

出　　处：《中国奶牛》2011年第6期4-7,共4页China Dairy Cattle

基　　金：现代农业(奶牛)产业技术体系建设项目(nycytx-0106)

摘　　要：以94头荷斯坦牛为试验材料,应用PCR-SSCP技术研究Leptin基因外显子2多态性与繁殖力的相关性。在Leptin基因外显子2的E2FB区上,经分析存在影响奶牛繁殖的多态位点,测序首次发现102nt处T突变为C。在平均产犊间隔上,AA与AT差异极显著(P<0.01);AT与TT差异极显著(P<0.01);A为优势等位基因,可作为荷斯坦牛育种中繁殖力的分子辅助标记。PCR-SSCP technology were employed to analyze the correlation between Leptin gene＇s polymorphism and fertility traits in 94 Holstein cows.The polymorphism（T/C）in the Locus 102bp affecting the cows＇ fertility was firstly detected in the exon 2 area （E2FB）of Leptin gene,after Sequencing.For the average parity interval,there were significant differences between genotype AA and genotype AT（P〈0.01）,between genotype AT and genotype TT（P〈0.01）.Genotype A was dominant allele,which could be applied for the molecular genetic markers in fertility of Holstein cows.

关 键 词：Leptin基因外显子2 胎间隔 繁殖力 荷斯坦牛 

分 类 号：S823[农业科学—畜牧学]