不同产地个青皮高效液相色谱指纹图谱研究  

HPLC Fingerprint of Fructus Citri Immaturu from Different Regions

作　　者：王桂红[1] 张远远[2] 肖伊[2] 胡俊杰[2] 郑国华[2] 

机构地区：[1]湖北中医药大学附属门诊部,武汉430065 [2]湖北中医药大学药学院,武汉430065

出　　处：《医药导报》2011年第4期429-431,共3页Herald of Medicine

基　　金：武汉市科技攻关计划(基金编号:200860423218)

摘　　要：目的研究不同产地个青皮的高效液相色谱(HPLC)法指纹图谱。方法用Aichrom Bond-AQ C18色谱柱(250 mm×4.6 mm,5μm),乙腈和0.1%磷酸水为流动相,采用梯度洗脱,流速:1.0 mL.min-1,柱温:30℃,检测波长:360 nm。结果建立HPLC指纹图谱共有模式,各产地个青皮药材质量有较好的相似性。结论该指纹图谱方法准确可靠,重复性好,可作为个青皮药材内在质量评价的依据。Objective To develop a high performance liquid chromatography（HPLC） fingerprint of Fructus citri immaturu from different regions. Methods The chromatographic column was Aichrom Bond-raising AQ C18（250 mm×4.6 mm,5 μm）.The mobile phase was acetonitrile and 0.1% phosphoric acid water with gradient elution.Flow rate was 1.0 mL·min-1at 30 ℃and detection wavelength was 360 nm. Results The common mode of HPLC fingerprint was developed and quality of Fructus citri immaturu from different regions was similar. Conclusion This method is accurate and reproducible,which can be used for the quality control of Fructus citri immaturu.

关 键 词：个青皮 指纹图谱 橙皮苷 色谱法 高效液相 

分 类 号：R282.71[医药卫生—中药学]