姜黄素对肝纤维化模型大鼠肝纤维化组织细胞因子的影响  

Effects of Curcumin on the Expression of Cytokines in Livers of Rats with Fibrosis

作　　者：张小斌 曾令兰[2] 陈莎燕[2] 王华[2] 

机构地区：[1]长江航运总医院、武汉脑科医院,430010 [2]华中科技大学同济医学院附属协和医院感染科,武汉430022

出　　处：《医药导报》2011年第4期443-446,共4页Herald of Medicine

摘　　要：目的了解姜黄素对实验大鼠肝纤维化组织中的基质蛋白酶-13(MMP-13)和金属蛋白组织抑制因子-1(TIMP-1)的干预。方法将22只雄性SD大鼠随机分为正常组(n=7)、模型组(n=6)和治疗组(n=9)。模型组和治疗组用四氯化碳制备大鼠肝纤维化模型,造模6周治疗组加用姜黄素,在10周末处死各组大鼠,取肝脏进行苏木精-伊红(hematoxylin and eosin,HE)染色,运用半定量逆转录聚合酶链反应(RT-PCR)方法检测MMP-13和TIMP-1的含量。结果 HE染色病理分级明显,治疗组与其他两组比较,HE染色病理分级高于正常组,低于模型组,差异均有统计学意义(P<0.05);而MMP-13mRNA的表达组间变化不大,但TIMP-1mRNA的含量在各组间差异有统计学意义(P<0.05),模型组和治疗组TIMP-1表达水平高于正常组,治疗组的TIMP-1mRNA表达与模型组比较明显下降(P<0.05)。结论姜黄素有抗纤维化作用,其机制可能是通过抑制大鼠肝脏中TIMP-1mRNA表达,减少胶原在肝脏内沉积,从而逆转和阻遏纤维化形成。Objective To observe the effects of curcumin on mRNA expression of matrix metal proteinase-13（MMP-13） and the tissue inhibitor of metal proteinase-1（TIMP-1） in hepatic tissues of experimental rats. Methods 22 male Sprague-Dawley rats were randomly divided into 3 groups ： normal group（n=7）,model group（n=6） and treatment group（n=9）.Hepatic fibrosis was induced by CCl4 in rats,which were treated with treatment after 6 weeks in the treatment group.All rats were sacrificed by the end of the 10th week,when HE staining of hepatic tissue was performed and the semi-quantitative polymerase chain reaction（RT-PCR） was used to assay mRNA expression of MMP-13 and TIMP-1. Results HE staining in the treatment group was significantly different from the model group（P0.05）;the mRNA expression of MMP-13 among each group was not remarkable different（P0.05）,but not for the mRNA expression of TIMP-1（P0.05）,Comparing to the normal group;TIMP-1mRNA in the model group and treatment group were obviously elevated（P0.05）,compared with the model group,while TIMP-1mRNA of the treatment group was reduced（P0.05）. Conclusion Curcumin reverses liver fibrosis,the mechanism of which associated to reducing the mRNA expression of TIMP-1,restoring the liver collagen degeneration system gradually.

关 键 词：姜黄素 肝纤维化 基质蛋白酶-13 金属蛋白酶组织抑制因子-1 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]