稻属叶绿体DNA限制性片段长度多态性  

Chloroplast DNA Restriction Fragment Length Polymorphism in the Genus Oryza

作　　者：朱世华[1] 王明全[2] 汪向明[2] 

机构地区：[1]宁波大学师范学院生物系,浙江宁波315211 [2]武汉大学生命科学学院遗传学系,湖北武汉430072

出　　处：《中国水稻科学》1999年第3期139-142,共4页Chinese Journal of Rice Science

基　　金：国家自然科学基金!38970440

摘　　要：对稻属（oryza）的14个种和李氏禾属（Leersia）的2个种共138份材料进行了叶绿体DNA限制性片段长度多态性的分析。用内切酶EcoRI酶切、4个叶绿体DNA探针杂交，共发现1o种叶绿体DNA变异类型。在稻属各个种内没有观察到叶绿体DNA的变异，叶绿体DNA变异类型基本上以稻属的染色体组型水平划分，因此认为精属的叶绿体DNA在进化过程中变异程度很低。推测CCDD组的o．alta、o．grandiglumis和o．latifolia及CC组的o．officinals和o.eichingeri各有共同的原始祖先。遗传距离分析显示CC、CCDD、BBCC组之间亲缘关系很近，这3个组与EE组亲缘关系也较近。李氏禾属的2个种L．perrieri和L．tisseranti与稻属的遗传距离很远。one hundred and thirty eight accessions of l4 species in the genus Oryza with two Leersia species were assayed for restriction fragment length polymorphism (RFLP) of the chloroplast DNA (cpDNA) using EcoR I and four probes of chloroplast DNA. Ten types of chloroplast DNA variation were observed. No polymophisms were detected within any speciesinvestigated. The classification of chloroplast genome types is in accordance with that of nuclear genomes of Oryza and it is inferred that variability of chloroplast DNA in genus Oryza is low. our data suggested that CCDD tetroploid species (o. alta,o.grandiglumis and o. latifolia ) and CC diploid species (o.of ficinalis and o. eichingeri ) with each other have a common ancestor while phylogenetic analysis indicated that CC,BBCC and CCDD genomes were closely related to one another andthey were rather closely related to EE genome a1so. Perrieri and tisseranti of genus Leersia have more far genetic distance fromgenus Oryza.

关 键 词：稻属 李氏禾属 叶绿体DNA 限制性片段长度 

分 类 号：S511.01[农业科学—作物学] S321