弓形虫诊断抗原的亲和层析纯化和分析  

Purification and determination of diagnostic toxoplasm:

作　　者：周永学[1] 王一成[1] 逄春泰[1] 

机构地区：[1]浙江省农业科学院畜牧兽医研究所

出　　处：《浙江农业学报》1999年第3期148-150,共3页Acta Agriculturae Zhejiangensis

摘　　要：应用琼脂糖凝胶Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析分离弓形虫速殖子细胞质抗原（Ｐｓ）和弓形虫速殖子膜抗原（Ｐｍ），分别获得亲抗体Ｐｓ和亲抗体Ｐｍ。经双多抗酶联免疫吸附试验（ＥＬＩＳＡ）夹心法测定，结果表明，Ｐｓ的最低检出量为１３．３μｇ／ｍｌ，亲抗体Ｐｓ为１．５μｇ／ｍｌ，Ｐｍ为４．４μｇ／ｍｌ，亲抗体Ｐｍ为０５μｇ／ｍｌ。作为弓形虫诊断抗原，亲抗体Ｐｓ和亲抗体Ｐｍ明显优于Ｐｓ和Ｐｍ，其中又以亲抗体Ｐｍ为最优。Cytoplasm antigen (Ps) and membrane antigen (Pm) of toxoplasm tachyzoites were parted by Sepharose 4B aflinity chromatography. Parental antibody Ps and parental antibody Pm were gained and determinated by ELISA.The minimal detectable concentration of Ps,parental antibody Ps,Pm and parental antibody Pm was 13.3 g/ml,1.5 g/ml,4.4 g/ml and 0.5 g/ml,respectively.As diagnostic antigen of toxoplasm,parental antibody Ps and parental antibody Pm were significantly superior to Ps and Pm,and parental antibody Pm was the best among them.

关 键 词：弓形虫 诊断抗原 纯化 

分 类 号：S852.72[农业科学—基础兽医学] S852.5[农业科学—兽医学]