水稻种子贮存醇溶蛋白4a基因的启动子Ⅰ及其调控元件  

Analysis of Promoter Ⅰ and Its Cis-acting Elements of Prolamin 4a Gene in Rice

作　　者：游志鹏[1] 廖玫江[1] 范云六[2] 

机构地区：[1]新疆农科院微生物研究所,乌鲁木齐830000 [2]中国农科院生物中心,北京100081

出　　处：《农业生物技术学报》1999年第2期103-108,共6页Journal of Agricultural Biotechnology

基　　金：洛克菲勒国际水稻生物技术资助!RF90032

摘　　要：水稻种子贮存醇溶蛋白4a基因5′端上游区由两个启动子组成。将启动子Ⅰ与报告基因GUS融合，在根癌农杆菌介导下，转化烟草，经组织化学分析，确定启动子Ⅰ为种子特异性启动子。启动于Ⅰ的5′端系列缺失分析证明，－212～－167区段是启动子Ⅰ种子特异性关键调控元件。The promoter of rice prolamin 4a gene was composed of promoter Ⅰ and promoter Ⅱ. The Promoter Ⅰ was fused to a β-glucuronidase (GUS) reporter gene and its expression evaluated in transgenic tobacco plant. HiStochemical analysis revealed that the promoter Ⅰ was a seed-specific promoter 5-promoter deletion analysis revealedthat a domain of the promoter Ⅰ, -212～- 167 from the translationalstart site, was a he}; ets-acting element for seed-specificexpression.

关 键 词：水稻醇溶蛋白 转录调控 启动子 水稻 种子贮存 

分 类 号：S511.041[农业科学—作物学]