小麦白粉病抗性基因Pm4a的RFLP标记转化为STS标记的研究  

Study on the Conversion of RFLP Markers co-segregated with Pm4a to Sequenced-tagged-site Markers

作　　者：刘金元[1] 刘大钧[1] 陶文静[1] 李万隆[1] 陈佩度[1] 

机构地区：[1]南京农业大学农业部作物细胞遗传重点开放实验室,南京210095

出　　处：《农业生物技术学报》1999年第2期113-116,共4页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金!39770413;国家‘863’计划!Z-17-04-01

摘　　要：RFLP标记Xbcdl231-2A与Pm4a基因共分离，按探针BCDl231两端的序列，设计出3个PCR扩增引物P1、P2、P3，并分别组成两对引物组合（P1＋P2；P2＋P3），以近等基因系为材料进行PCR扩增，当退火温度在45～50℃之间时，引物组合P1＋P2在抗病NILs中扩增出一条约为1．7kb大小的特异带，而感病亲本中则不具此带。进一步对11个含Pm4a或Pm4b及7个不含Pm4基因的材料进行扩增，结果发现，此1．7kb特异带存在于所有11个含Pm4基因材料中，但所有7个不具Pm4基因材料中则扩增不出此带。该STS标记与Pm4基因连锁的紧密程度尚待以相应F2分离群体作进一步确定。Based on the sequence data ofclone BCD 1231, a pair of 23-hp primers were designed. When the annealing temperature is 45～50℃, a specific band of about 1 .7 kb was amplified from samples containing Pace4a gene. Further amplification using the primers found the specific band was only present in the tasted cultivars and lines containing Pm4a and Pm4b genes, but absent in the gCnotypes without Pm4 gene. As PCR-based markers may be of greater interest of breeders, further studies on PCRbased markers are proceeding.

关 键 词：RFLP标记 Pm4a基因 PCR扩增 STS标记 小麦白粉病 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]