CliniMACS系统进行CD^＋34造血干细胞阳性富集的应用评价  

Evaluation of transplantation with CD＋34 hematopoietic stem cells enrichment by using CliniMACS device

作　　者：吴书一 董秀娟 刘文刚 赛亚 朱趁红 赵晓武 

机构地区：[1]第三人民医院干细胞与器官移植实验室,郑州市450000

出　　处：《中国实用医刊》2011年第7期1-2,5,共3页Chinese Journal of Practical Medicine

基　　金：河南省自然科学基金项目（0411045200）;河南省科委科技发展计划项目（001170610）

摘　　要：目的探讨CliniMACS系统进行CD^＋34造血干细胞阳性富集的有效性、安全性及应用效果。方法对39例实体瘤和自身免疫性疾病患者动员后采集的外周血干细胞进行了CliniMACS系统阳性富集的CD^＋34自体造血干细胞移植。结果阳性富集后CD^＋34细胞纯度平均可达89．8％，CD^＋34细胞平均回收率达78．9％，粒细胞-巨噬细胞集落形成单位（CFU—GM）数量为（15．0±7．0）×10^5／kg，阳性富集前、后细胞胎盘蓝拒染率比较差异无统计学意义，保持了细胞良好的生物学活性，移植后于2～3周内造血重建。结论应用CliniMACS系统进行CD^＋34造血干细胞阳性富集，可安全、有效地应用于造血干细胞移植。Objective To investigate the validity and security of CD ＋ 34 hematopoietic stem cells enrichment by using CliniMACS device. Methods Thirty -nine patients with solid tumor and autoimmune disease received CD＋34 HSCT, the CD ＋ 34 cells were enriched by CliniMACS device. Results After en- richment, the median purity of CD ＋ 34 cells was 89.8%, the median recovery rate of CD ＋ 34 cells was 78.9%, the granulocyte - macrophage cluster - colony forming unit （ CFU - GM） counts were （ 15.0±7.0）×105/kg. The refuse rate of typan blue was similar, compairing before and after enrichment, and the hematopoietic function recovers 2 -3 weeks after transplantation. Conclusions The feasibility and safety of the CD ＋ 34 cells enrichment by using CliniMACS device in patient before HSCT were confirmed.

关 键 词：CD+34细胞 阳性富集 造血干细胞移植 CliniMACS 

分 类 号：R457.7[医药卫生—治疗学]