异种脱细胞真皮替代睑板材料重建眼睑的可行性  

Feasibility of eyelid reconstruction with acellular xenogenic dermal matrix

作　　者：张向荣[1] 周琼[1] 肖卫[2] 刘德伍 彭燕 

机构地区：[1]南昌大学第一附属医院眼科,江西省南昌市330006 [2]南昌大学第一附属医院烧伤研究所,江西省南昌市330006 [3]佛山市祥城区张槎医院眼科,广东省佛山商528000

出　　处：《中国组织工程研究与临床康复》2011年第3期395-398,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：江西省卫生厅科技计划(20071033),课题名称:异种脱细胞真皮在眼睑重建术中的实验及临床研究~~

摘　　要：背景:眼睑后层重建是眼睑重建的重点和难点,其中睑板替代物更是研究的焦点。异种脱细胞真皮作为一种新型的组织工程材料,在国内外烧伤整形领域,正得到广泛的研究和应用。目的:观察异种(猪)脱细胞真皮植入兔眼睑后的组织相容性极其组织病理学变化。方法:剥取健康小白猪全层皮肤20cm×20cm,制备异种(猪)脱细胞真皮基质。同时制备兔睑板全层缺损模型并植入脱细胞真皮基质,观察大体情况,并分别于第1,2,3周取移植交界处眼睑组织光镜下观察组织学的改变。结果与结论:大体观察未见明显排斥反应及眼睑的变形;光镜下1周时可见局部炎症细胞浸润,2周时炎症细胞减少,3周时正常纤维组织长入,逐渐分割代替植入的胶原纤维,炎症反应消失。提示异种脱细胞真皮免疫原性低,并可引导新生胶原的生长,是一种良好的睑板替代物。BACKGROUND： Reconstruction of posterior lamella of eyelid is an important and difficult issue in eyelid reconstruction, and tarsal substitute is the focus of the study. Xenogenic acellular dermal matrix as a new tissue engineering material, is being widely studied and applied in the field of burn and plastic surgery at home and abroad. OBJECTIVE： To observe histocompatibility and histopathological changes of xenogenic （porcine） acellular dermal matrix transplantation for eyelid reconstruction in rabbits. METHODS： Full skin at 20 cm×20 cm was stripped from healthy little white pigs, for preparation of xenogenic （porcine） acellular xenogenic dermal matrix. Model of rabbit tarsal defect was established in 8 New Zealand rabbits, then acellular dermal matrix was implanted. Samples of implanted materials were collected for histological examination at 1, 2, 3 weeks postoperation under light microscopy. RESULTS and CONCLUSION： There were no obvious rejection and eyelid deformation observed. One week after implantation, partial inflammatory cells invasion was seen with light microscopy, and the inflammatory cells were reduced noticeably at 2 weeks after implantation, at 3 weeks after implantation, normal fibrous tissue ingrew and gradually split, instead of collagen fibers implanted, the inflammation disappeared. With low immunogenicity, and guidance for the growth of new collagen, the xenogenic acellular dermal matrix may be an ideal material of tarsal substitbutes.

关 键 词：异种脱细胞真皮 眼睑重建 组织工程 材料 相容性 

分 类 号：R318[医药卫生—生物医学工程]