壳聚糖球形多孔微载体的血液相容性评价  

Blood compatibility evaluation of the spherical porous chitosan microcarrier

作　　者：张瑞[1] 韩宝三[2] 彭承宏[2] 

机构地区：[1]内蒙古医学院第一附属医院肿瘤外科,内蒙古自治区呼和浩特010059 [2]上海交通大学医学院附属瑞金医院器官移植中心,上海市200025

出　　处：《中国组织工程研究与临床康复》2011年第3期404-408,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家高新技术研究发展计划"863"项目(2007AA02Z487);课题名称:微粒生物人工肝反应器构建的研究;国家自然科学基金项目(30672043);课题名称:微粒肝细胞生物反应器-微囊化三维多孔微载体构建的研究;国家自然科学基金项目(30772105);课题名称:新型超分子自组装微囊/肝细胞球形聚集体构建的研究;国家自然科学基金项目(20074031);课题名称:人肝细胞/聚合物杂化界面和生物人工肝的研究~~

摘　　要：背景:文献报道的微载体大多为实心的、大孔的,虽然较二维微载体的比表面积有明显的增加,但距理想的三维微环境相差甚远。目的:构建壳聚糖球形多孔微载体,通过溶血实验、凝血实验、血小板计数及聚集实验评价其血液相容性。方法:利用液氮冷冻干燥技术成功构建浓度为1%,2%,3%的壳聚糖球形多孔微载体。选择健康成年新西兰兔为宿主,采用溶血实验、凝血实验、血小板计数及其聚集实验评价壳聚糖球形多孔微载体的血液相容性。结果与结论:浓度为1%,2%,3%的壳聚糖球形多孔微载体的溶血率分别为1.56%,2.07%,2.31%,均小于5%,均无致溶血性;3种浓度壳聚糖球形多孔微载体样本材料对兔血时间无明显影响,三者与生理盐水阴性对照组间也无明显差别(P>0.05);3种浓度壳聚糖球形多孔微载体样本材料对兔血小板计数无明显影响,注入浸提液前后比较和组间比较差异均无显著性意义(P>0.05)。证实壳聚糖球形多孔微载体无致溶血性、无凝血性和无血小板聚集性,表明壳聚糖球形多孔微载体具有良好的血液相容性。BACKGROUND： Literature reported that most of microcarrier was solid and macroporous. Although surface area was significantly increased compared with two-dimensional microcarrier, it was far away from ideal three-dimensional environment. OBJECTIVE： To construct spherical porous chitosan microcarrier, and to evaluate the blood compatibility of the spherical porous chitosan microcarrier by hemolysis, coagulation, platelet count, platelet aggregation tests. METHODES： The concentration of 1%, 2%, and 3% spherical porous chitosan microcarrier were successfully constructed using liquid nitrogen freeze-drying technology. Healthy, adult, New Zealand Rabbits were selected, the blood compatibility of spherical porous chitosan microcarrier was evaluated by hemolysis, coagulation, platelet count, platelet aggregation tests. RESULTS AND CONCLUSION： The concentration of 1%, 2%, and 3% spherical porous chitosan microcarrier hemolysis rate was1.56%, 2.07%, 2.31%, less than 5%, and without hemolyticus. 3 spherical porous chitosan microcarrier concentrations of sample materials had no significant effect on rabbit blood time. There was no significant difference between 3 concentration of spherical porous chitosan microcarrier and sodium chloride negative control group （P 0.05）. 3 spherical porous chitosan microcarrier concentrations of sample materials had no significant effect on platelet count. There was no significant difference between the comparison of leaching liquor injection before and after and intragroup comparison （P 0.05）. The results have confirmed that the spherical porous chitosan microcarrier has no hemolyticus, coagulation, and platelet aggregation. It shows that the spherical porous chitosan microcarrier has good blood compatibility.

关 键 词：人工肝脏 壳聚糖 微载体 血液相容性 组织工程 

分 类 号：R318[医药卫生—生物医学工程]