二袖套法建立恒河猴原位肝移植的稳定模型  

Construction of stable rhesus monkey orthotopic liver transplantation models using two-cuff technique

作　　者：冉江华[1,2] 李铸[1,2] 刘静[1,2] 张升宁[1,2] 吴淑媛[1,2] 张熙冰 李来邦[1,2] 李立[1,2] 张鸿青[1,2] 刘滇生[1,2] 

机构地区：[1]昆明市第一人民医院肝胆胰一病区 [2]昆明医学院附属甘美医院,云南省昆明市650011

出　　处：《中国组织工程研究与临床康复》2011年第5期762-768,共7页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：昆明市科技局重大项目(08S100304);项目名称:昆明器官移植研究中心建设;子课题名称:肝移植急性排斥反应早期诊断的实验研究~~

摘　　要：背景:以往多应用大鼠建立肝移植模型对肝移植术后排斥反应进行基础研究,而灵长类非人类大动物建立肝移植模型能更接近临床肝移植的需求。目的:探讨建立稳定的恒河猴原位肝移植模型的最佳方案。方法:选用健康恒河猴进行同种异体原位肝移植20次,以10只雄性作为受体,10只雌、雄不限为供体,借鉴临床肝移植和各种动物模型建立的方法,使用二袖套法加肝动脉重建建立稳定的恒河猴原位肝移植模型。结果与结论:20次恒河猴原位肝移植模型成功率90%,供肝切取时间(17±3)min,供肝修整时间(35±5)min,受体移植时间(133±45)min,受体无肝期(12±4)min,术后24h存活率90%(18/20),72h存活率为80%(16/20),1周存活率50%(10/20),14只分别于术后2周内死于急性排斥反应,最长存活38d,也死于急性排斥反应,所有受体均无门静脉血栓形成及胆道并发症发生。改进后的恒河猴同种异体原位肝移植模型具有操作简便、手术成功率高、重复性和稳定性好的优点,是肝移植临床前期研究的较理想动物模型。BACKGROUND：Rat orthotopic liver transplantation usually used as basic animal model for liver transplantation acute rejection research. But for human,it is wised to use nonhuman primates to establish this model and obtain some experiment results matched for clinical needs in this field. OBJECTIVE：To explore improvement of orthotopic liver transplantation model in rhesus monkey. METHODS：Healthy rhesus monkeys were chosen to perform orthotopic liver transplantation for 20 times. The model was established by drawing on a variety of animal model methods plus clinical liver transplantation experiences,and two cuff technique,hepatic artery reconstruction were used to establish stable model of orthotopic liver transplantation in rhesus monkeys. RESULTS AND CONCLUSION：Twenty orthotopic liver transplantation models in rhesus were performed,and the achievement ratio of operation was 90%. The mean liver graft procurement time,donor Liver preparation time,recipient operation time and anhepatic phase was （17±3） minutes,（35±5） minutes,（133±45） minutes and （12±4） minutes,respectively. The survival rate at24 hours after operation was 90% （18/20）,at 72 hours was 80% （16/20）,and at 1 week was 50% （10/20）.14 rhesus monkeys died of acute rejection within 2 weeks after operation. The longest survival time was 38 days and this recipient also died of acute rejection. All recipients were without portal vein thrombosis and biliary complications. The improved rhesus monkey model of orthotopic liver transplantation is easy to perform with high achievement ratio of operation,superior reproducibility and stability. It is an ideal animal model for pre-clinical studies of liver transplantation.

关 键 词：二袖套法 肝移植 动物模型 恒河猴 血管重建 

分 类 号：R617[医药卫生—外科学]