高效液相色谱法测定萨拉沙星  

Determination of Sarafloxacin by High Performance Liquid Chromatography (HPLC)

作　　者：何娟[1] 常使标 刘澎[1] 郭瑞云[1] 林素凤[1] 邝爱燕[1] 

机构地区：[1]河南省分析测试中心,郑州450002

出　　处：《色谱》1999年第4期393-394,共2页Chinese Journal of Chromatography

摘　　要：采用高效液相色谱法测定了萨拉沙星。色谱柱为μ－BondapakTMC18柱（3．9mm×300mm），流动相为V（乙腈）：V（甲醇）：V（2mmol／L磷酸，用三乙胺调pH3．5）＝30：5：65，用二极管阵列检测器检测，检测波长278nm，得到了满意的分离效果。A method for the separation and determination of Sarafloxacin by HPLC was developed. The sample was dissolved in a mixture of V (acetonitrile): V (water)=1 : 1. Operating conditions were as follows:μ-Bondapak TM C18 column (3. 9 mm× 300 mm), V (acetonitrile): V (methanol): V (2 mmol/L H3PO4, adjust PH 3. 5 with triethylamine) = 30: 5: 65 as mobile phase with a flow rate of 1 mL/min, UV detection at 278 um, and column temperature was 15℃. Under the above conditions, Sarafloxacin and other impurities: were separated from each other. The method was simple, rapid, sensitive and accurate.

关 键 词：萨拉沙星 紫外检测 HPLC 测定 

分 类 号：S859.79[农业科学—临床兽医学]