急性敌敌畏中毒大鼠的早期心肌损伤  

The myocardial injury in the early stage of acute dichlorvos poisoning in rats

作　　者：汤罗嘉[1] 姚晨玲[1] 黄培志[1] 

机构地区：[1]复旦大学附属中山医院急诊科,上海200032

出　　处：《中华急诊医学杂志》2011年第3期273-276,共4页Chinese Journal of Emergency Medicine

基　　金：国家科技支撑计划专题基金资助（2006BAl06B01）

摘　　要：目的研究急性敌敌畏（dichlorvos，DDVP）中毒导致的早期心肌损伤表现。方法SpragueDawley（sD）大鼠24只，随机（随机数字法）分成对照组和中毒组两组，每组12只。采用右侧颈动脉置管术动态监测有创血流动力学指标；测定血清肌钙蛋白T（cardiac troponin T，CTnT）和心房利钠肽（brain natriuretic peptide，BNP）水平；观察心肌组织病理学改变。结果①中毒组自身前后对照：中毒1h内，心率（heart rate，HR）和室内压上升段最大变化率（maximum ascendin grates of left ventricular pressure，＋dp／dtmax）均有显著下降；室内压下降段最大变化率（maximum descending rates of left ventricular pressure，-dp／dtmax），左室舒张末压（left ventricular end diastolic pressure，LVEDP）有显著升高（P〈0．01）。其中均以7min时改变最显著。②中毒组与对照组比，血清CTnT值显著升高（P〈0．01），血清BNP值差异无统计学意义（P〉0．05）。③中毒组心肌HE染色存在病理学损害。结论急性有机魄中毒（acute organophosphorus pesticides poisoning，AOPP）其早期可导致大鼠心肌损伤，血流动力学紊乱，心功能受抑。CTnT可作为早期评价AOPP心肌损伤的指标，而BNP在AOPP诊治中的意义不大。Objective To investigate the myocardial injury in the early stage of acute diehlorvos poisoning in rats. Method A total of 24 Sprague Dawley rats were randomly （ random number） divided into control group（ n = 12） and poisoning group（ n = 12）. Hemodynamic variables were monitored by using an arterial cannula inserted into right arteria carotis communis. Serum levels of cardiac troponin T （CTnT） and brain uatriuretic peptide （BNP） were measured. Myocardial tissue was observed with HE stain under microscope. Results The rats of poisoning group showed that the heart rate （HR） and maximum ascending rates of left ventricular pressure （ ＋ dp/dtmax ） were significant decreased in an hour after poisoning （P 〈 0.01 ）. The maximum descending rates of left veutricular pressure （ - dp/dtmax ） and left ventricular end diastolic pressure （LVEDP）were markedly increased （P 〈0.01） and reached peak in 7 minutes in the poisoning group. Compared with the control group, cardiac troponin T obviously changed in rats poisoned with dichlorvos in the first hour. BNP was not affected after poisoning（P 〉 0.05）. Myocardial damage was found in the poisoning rats. Conclusions Myocardial injury and heart failure occurred in the early stage of acute organophosphorus pesticides poisoning（AOPP） in rats. CTnT could play a major role in AOPP while BNP might not be involved in.

关 键 词：急性有机磷中毒 敌敌畏 中毒 大鼠 血流动力学 肌钙蛋白T 心房利钠肽 心肌损伤 

分 类 号：R595.4[医药卫生—内科学]