阿奇霉素对支气管哮喘小鼠气道重构及转化生长因子-β1表达的影响  

Effects of azithromycin on airway remodeling and transforming growth factor-β1 in asthmatic mice

作　　者：郑美梅[1] 江勇[1] 温武金[1] 王方剑[1] 段成城[1] 潘小华[1] 董丽妍[1] 

机构地区：[1]广州医学院附属深圳沙井医院呼吸内科,深圳518104

出　　处：《中国医师杂志》2011年第2期158-161,共4页Journal of Chinese Physician

基　　金：广东省深圳市宝安区科技局资助项目（2010623）

摘　　要：目的 观察阿奇霉素对哮喘小鼠气道重构指标、转化生长因子-β1（transforming growth factor-β1,TGF-β1）表达的影响,探讨阿奇霉素对哮喘小鼠气道重构的影响.方法 30只雄性BALB/c小鼠随机分为正常对照组（A组）、哮喘模型组（B组）、阿奇霉素治疗组（C组）,每组各10只,复制哮喘模型后收集支气管肺泡灌洗液（BALF）,行白细胞（TOT）,嗜酸性粒细胞（EOS）计数;医学图像分析软件观测支气管总管壁面积（Wat）、内壁面积（Wai）、平滑肌面积（Wam）;SABC免疫组化法检测肺组织TGF-β1的表达.结果 B组BALF中嗜酸性粒细胞明显高于其他组（8.12±0.54 vs 0.70±0.40,8.12±0.54 vs 0.87±0.25,P＜0.01）;C组气道Wat、Wai、Wam均较B组减轻（10.15±0.95 vs 15.36±0.85,4.16±0.32 vs 10.64±1.03,3.77±0.15 vs 7.97±0.17,P＜0.01）,但较A组加重;B组TGF-β1（168.58±21.71 vs 130.47±18.22 vs 126.30±16.15）表达最强,C组减弱,A组微弱;TGF-β1的表达与EOS%（r=0.840,P＜0.01）、Wat（r=0.735,P＜0.01）、Wam（r=0.870,P＜0.01）呈正相关.结论 阿奇霉素可抑制哮喘小鼠气道重构,可能是通过抑制TGF-β1的表达来实现的.Objective To investigate the effects of azithromycin on airway remodeling and the expression of transforming growth factorβ1 （TGF-β1） in asthmatic mice.Methods BALB/c male mice were random divided into 3 groups：control group（A）,asthma group （B）,and azithromycin treated group （C）,with 10 mice in each group.Bronchoalveolar lavage fluid was collected to count the number of white blood cells and eosinophilic granulocytes EOS.The morphological parameters of the bronchi were measured by computer image analysis and the pathologic changes of the bronchi and lung tissue were observed by HE staining.The expressions of TGF-β1 were detected by immunohistochemistry.Results The number of EOS in B group was significantly higher than that in control group（8.12 ±0.54 vs 0.70 ±0.40;8.12 ±0.54 vs 0.87 ±0.25,P 〈0.01）.WAt and WAi and WAm in group C was significantly lower than that in group B （10.15 ±0.95 vs 15.36 ±0.85,4.16 ±0.32 vs 10.64 ± 1.03,3.77 ±0.15 vs 7.97 ±0.17,P 〈0.01）but higher than that in group A.The expression of TGF-β1 in group C was significantly lower than that in group B but higher than that in group A.TGF-β1 expression of lung tissue in C group was significantly correlated with EOS,（r =0.840,P 〈0.01） and WAt（r =0.735,P 〈0.01） and WAm （r =0.870,P 〈0.01）.Conclusion Azithromycin inhibited airway remodeling in asthmatic mice,which might possibly be achieved through inhibiting the expression of TGF-β1.

关 键 词：叠氮红霉素/药理学 哮喘/药物疗法/代谢/病理生理学 气管/病理生理学/药物 作用 转化生长因子β1/代谢 

分 类 号：R562.25[医药卫生—呼吸系统]