IGF-1基因启动子区CA重复序列多态性与代谢综合征关系研究  

Association of CA repeats polymorphism in the promoter region of IGF-1 gene with metabolic syndrome

作　　者：杨敏[1] 向红丁[1] 陈伟[1] 凌伟[1] 

机构地区：[1]天津医科大学代谢病医院心内科,卫生部及天津市激素与发育重点实验室,天津300070

出　　处：《中国医师杂志》2011年第2期197-199,202,共4页Journal of Chinese Physician

摘　　要：目的 探讨胰岛素样生长因子1（IGF-1）基因启动子区CA重复序列多态性与代谢综合征（MS）的关系.方法 收集北京市东城区常住人口1047例,采用2005年国际糖尿病联盟标准诊断代谢综合征.将受试者的基因组DNA应用聚合酶链反应扩增,再通过选择不同长度的纯合子样本测序确定CA重复的次数,以确定等位基因.同时测量身高、体重、腰围、血糖、血脂、胰岛素及血IGF-1.结果 （CA）19纯合组MS患病率显著低于不携带（CA）19组（9.1% vs 24.0%,Х^2=18.05,P＜0.01）及（CA）19杂合组（9.1% vs 18.3%,Х^2=8.55,P＜0.01）;三组间血IGF-1水平差异有统计学意义[（114.0±52.6）μg/L vs（136.6±80.5）μg/L vs（129.2±49.1）μg/L,F=3.16,P＜0.05],（CA）19纯合组血IGF-1水平低于不携带（CA）19组及（CA）19杂合组.三组间体重指数（BMI）、腰围（WC）、甘油三酯（TG）、空腹胰岛素（Fins）、2hIns及胰岛素敏感性指数（ISI）差异均有统计学意义（P＜0.05）,（CA）19纯合组的BMI、WC、TG、FIns、2hIns均低于另两组,而ISI高于另两组.结论 IGF-1基因启动子区CA重复序列多态性与汉族人群MS发病有关.Objective To explore the relationship between CA repeats polymorphism in the promoter region of IGF-1 gene and MS in the Han nationality.Methods 1047 subjects were recruited from general population of Dongcheng District in Beijing.MS was diagnosed based on the criteria for MS in 2005 by IDF.Genomic DNA was extracted by standard methods.PCR,Genescan,Genotyper and direct sequencing were conducted to screen CA repeats polymorphism in the promoter region of the human IGF-1 gene.Levels of plasma glucose,lipids,serum insulin and IGF-1 were determined.BMI and ISI were calculated.Results The prevalence of MS in （CA） 19 homozygote was lower than that in （CA） 19 heterozygote （9.1% vs 18.3%,Х^2 = 8.55,P 〈 0.01） and without （CA） 19 （9.1% vs 24.0%,Х^2 = 18.05,P 〈 0.01）.The level of serum IGF-1 had differences among the three groups [ （114.0 ± 52.6） μg/L vs （136.6 ± 80.5） μg/L vs （129.2±49.1） μg/L,F =3.16,P 〈0.05],（CA）19 homozygote had lower serum IGF-1 than （CA）19heterozygote and without （CA） 19.BMI,WC,TG,FIns,2hIns and ISI had difference among the three groups （P 〈0.05）.Conclusions （CA）19 repeats polymorphism in the promoter region of IGF-1 gene was significantly associated with MS in Han nationality.

关 键 词：胰岛素样生长因子I/遗传学 多态现象 遗传 启动区(遗传学) 代谢综合征X/遗传学 

分 类 号：R589[医药卫生—内分泌]