GCR1和GCR2在N-酰基高丝氨酸内酯调节拟南芥根生长中的作用  

Involvment of GCR1 and GCR2 in the Regulation of Root Growth by N-Acyl-Homoserine Lactones in Arabidopsis thaliana L.

作　　者：边子睿[1,2] 刘方[1] 张霞[1] 张哲[1,2] 宋水山[1] 

机构地区：[1]河北省科学院生物研究所,石家庄050051 [2]河北工业大学化工学院,天津300130

出　　处：《植物生理学报》2011年第2期175-180,共6页Plant Physiology Journal

基　　金：国家重点基础研究发展计划(2009CB126000);河北省自然科学基金(C2006000707);河北省重点基础研究计划项目(08965506D)

摘　　要：N-酰基高丝氨酸内酯(AHLs)是革兰氏阴性细菌群体感应的信号分子。培养基中添加1μmol·L-13OC6-HSL和10μmol·L-13OC8-HSL可显著促进野生型拟南芥主根生长,但拟南芥G蛋白偶联受体GCR1和GCR2基因缺失突变体gcr1-1和gcr2-2对AHLs处理不敏感;实时荧光定量PCR分析显示,这2种AHLs的处理可以使拟南芥GCR1和GCR2基因表达量上调2～4倍。结果表明,G蛋白偶联受体GCR1和GCR2可能参与植物感应细菌信号进而做出根生长响应的信号转导。N-acyl-homoserine lactones（AHLs） is the quorum-sensing signal molecular in Gram-negative bacteria.Addition of 1μmol·L-1 3OC6-HSL or 10μmol·L-1 3OC8-HSL in the medium significantly promoted the root growth of wild type Arabidopsis thaliana.However,the two Arabidopsis mutants gcr1-1 and gcr2-2 lacking GCR1 and GCR2 respectively showed insensitive to these two signals.Real Time PCR analysis showed that the expression levels of GCR1 and GCR2 are increased 2–4 times by the treatment of these two AHLs.These results indicated that GCR1 and GCR2 might be involved in the signal transduction in Arabidopsis root response to bacterial quorum-sensing signals.

关 键 词：G蛋白偶联受体 N-酰基高丝氨酸内酯 拟南芥 GCR1 GCR2 

分 类 号：Q946[生物学—植物学]