金属螯合亲和膜吸附分离与纯化溶菌酶的研究(Ⅱ)──不同金属螯合亲和膜对溶菌酶的吸附性能  

Studies on the Affinity Membranes for Adsorption and Purification of Lysozyme(Ⅱ) Adsorption Characteristics of Metal Chelating Affinity Membranes for Lysozyme

作　　者：李静[1] 陈欢林[1] 柴红[1] 

机构地区：[1]浙江大学化工学院,杭州310027

出　　处：《高等学校化学学报》1999年第8期1322-1327,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金! (批准号 :2 96760 38)资助课题

摘　　要：采用低温氧或氨等离子体法改性聚丙烯微孔膜 ,基于等离子体改性膜的活化、偶联及螯合过程的机理 ,制备了 Fe3+,Ni2 +,Cu2 +和 Zn2 +等金属离子螯合亲和膜 ,并用于溶菌酶的吸 -脱附实验 .实验结果表明 ,Ni2 +和 Cu2 +离子螯合亲和膜对溶菌酶具有较高的吸附量 ,螯合过程中采用氯化物盐溶液制得的膜对溶菌酶吸附量比采用硫酸盐溶液制得的膜的吸附量高 .两种膜的重复吸-脱附性能相近 ,而 Fe3+螯合亲和膜基本上不能用于重复吸 -脱附实验 .采用补充金属螯合离子 ,能部分恢复亲和膜对溶菌酶的吸附量 ,是实现亲和膜重复使用的有效方法 .Based on activation, coupling and chelating processes, PP(polypropylene) microporous membranes whose surface was treated by low temperature plasma were used for preparation of affinity membranes with Fe 3+ , Ni 2+ , Cu 2+ and Zn 2+ metal chelating ligand respectively. Those affinity membranes have been used for adsorption desorption experiments of lysozyme. The experimental results showed that adsorption capacity of the membranes with Ni 2+ or Cu 2+ metal chelating ligand is higher than that with Fe 3+ or Zn 2+ metal chelating ligand. The adsorption capacity of affinity membrane made in Cl - ion solution is also higher than that made in SO 2- 4 ion solution. The experimental results also showed that affinity membranes with Ni 2+ or Cu 2+ can be reused several times while affinity membrane with Fe 3+ ion can not. Adsorption amount of lysozyme can be partly restored by addition of metal ions on the reused affinity membranes.

关 键 词：聚丙烯膜 亲和吸附 溶菌酶 膜分离 亲和膜 纯化 

分 类 号：O658.68[理学—分析化学] Q550.3[理学—化学]