PPAR-γ对放射诱导成纤维细胞分泌PAI-1和TGF—β的影响  

Influence of PPAR - γ ligand on radiation - induced PAI - 1 and TGF -β mRNA in fibroblast cells

作　　者：张一思[1] 张大昕[1] 

机构地区：[1]哈尔滨医科大学附属第一医院肿瘤一科,150001

出　　处：《中华放射肿瘤学杂志》2011年第2期168-171,共4页Chinese Journal of Radiation Oncology

基　　金：国家自然科学基金资助项目（30471973）

摘　　要：目的分析过氧化物酶增殖体活化受体．7（PPAR－7）配体（匹格列酮）对x射线诱导纤溶酶原抑制物-1（PAI-1）、转化生长因子－β（TGF—β）在小鼠肺成纤维细胞（L929细胞）中的mRNA表达和L929细胞增殖活性的影响。方法用RT-PCR证实L929细胞表达PPAR-γmRNA。分别采用10、4、2GyX射线照射L929细胞，观察照射前后PAI-1、TGF-13mRNA的表达情况。照射＋匹格列酮处理L929细胞，用RT—PCR方法观察对PAI-1、TGF-13mRNA的表达影响，用MTF法观察对细胞增殖活性影响。结果RT-PCR检测结果显示10Gy照射48h后L929细胞中PAI-1、TGF-13mRNA表达明显升高[0．18：0．78、0．22：0．76（F=3．70，P=0．010）]，2Gy和4Gy有类似趋势[0．18：0．43、0．18：0．44和0．22：0．39、0．22：0．40（F=3．40，P=0．090）]；10Gy照射＋匹格列酮48h后明显降低[0．78：0．45、0．76：0．54（F=3．90，P=0．010）]，2Gy和4Gy的也有类似趋势[0．43：0．37、0．44：0．35和0．39：0．32、0．40：0．32（F=2．40，P=0．210）]。Mqq＂结果显示与空白对照比较2、4、10Gy照射和单用匹格列酮均会抑制L929细胞增殖[0．44：0．39、0．36、0．34和0．32（F=3．90，P=0．040）]，2、4、10Gy照射＋匹格列酮更能抑制L929细胞增殖[0．44：0．27、0．26、0．25（F=2．50，P=0．005）]。结论 X射线照射L929细胞后出现促纤维化因子PAI-1、TGF-p表达升高。匹格列酮使PAI-1、TGF—p高表达降低，也会抑制成纤维细胞的增殖。Objective To observe the influence of peroxisome proliferator activated receptor-γligand （PPAR-γ, pioglatazone） on expression of PAI-1 and TGF-β mRNA and proliferation in fibroblast cells before and after X-ray radiation, and to study the effect of PPAR-γ on normal ceils during radiation induced fibrosis process. Methods RT-PCR method was used to measure PPAR--,/gene expression in L929 cells. After X-ray irradiation of 10 Gy,4 Gy or 2 Gy, the expressions of PAI-1 and TGF-β mRNA in mouse lung fibroblast cells （L929） were measured using RT-PCR. After X-ray irradiation and pioglatazone treatment, the influence of pioglatazone on PAI-1 and TGF-[3 was measured using RT-PCR method. MTF method was used to test cell proliferation after the treatment of irradiation and pioglatazone. Results PPAR-~ mRNA expression was observed in L929 cells. Expression of PAI-1 and TGF-[3 mRNA reached the highest level 48 h after radiation of 10 Gy （ 0. 18 ： 0.78, and 0. 22 ： 0. 76 （ F = 3.70, P = 0. 010 ） ）. Similar results were obtained under radiation of 2 Gy and 4 Gy （0. 18 ： 0. 43, 0. 18 ： 0. 44, and O. 22： 0. 39, 0. 22 ： 0. 40 （ F = 3.40, P = 0. 090） ）. At 48 h after the treatment of pioglatazone and 10 Gy radiation, pioglatazone decreased the level of radiation-induced PAI-1 and TGF-~ in L929 cells （0.78： 0. 45, and 0.76： 0. 54 （F = 3.90,P = 0. 010） ）. Similar trend was observed under radiation of 2 Gy and 4 Gy （0.43： 0. 37, 0.44： 0. 35, and 0. 39 ： 0. 32, 0. 40 ： 0. 32 （ F = 2.40, P = 0. 210） ）. Compared with the control group, the proliferation of L929 cells was inhibited by X-ray radiation of 2 Gy, 4 Gy and 10 Gy, or by pioglatazone alone （0. 44： O. 39, 0. 36, 0. 34 and 0. 32（ F = 3.90 ,P = 0. 040） ）. The inhibitory effect was significantly increased when L929 cells were treated by pioglatazone and radiation of 2 Gy,4 Gy, or 10 Gy （0. 44： 0. 27, 0. 26, and 0. 25 （ F =2. 50,P =0. 005））. Conclusions X-ray irradiation can increase the expression

关 键 词：过氧化物酶增殖体活化受体-γ配体 纤溶酶原抑制物-1基因 转化生长因子-β基因 L929细胞 照射 

分 类 号：R285.5[医药卫生—中药学]