IRF-4结合蛋白对人涎腺腺样囊性癌细胞增殖的抑制作用  

Inhibitory effect of IRF-4 binding protein on proliferation of human salivary adenoid cystic carcinoma cells

作　　者：熊剑[1,2] 简从相[1] 李鹏[3] 范舒[3] 申涛[1] 常慧君[1] 胡川闽[3] 周继祥[1] 

机构地区：[1]第三军医大学西南医院口腔科 [2]咸宁学院口腔系,湖北咸宁437100 [3]第三军医大学临床生物化学教研室,重庆400038

出　　处：《第三军医大学学报》2011年第6期554-557,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30901458);重庆市自然科学基金(CSTC2009BB5027)~~

摘　　要：目的探讨IRF-4结合蛋白(IRF-4 binding protein,IBP)对人涎腺腺样囊性癌细胞增殖的影响。方法用免疫组化(S-P法)染色技术对27例人涎腺腺样囊性癌组织和20例正常涎腺组织标本进行IBP表达的检测和分析。用脂质体法转染IBP过表达及空白对照载体至人腺样囊性癌细胞株ACC2,G418筛选后建立IBP稳定表达细胞株;MTT法和平板克隆形成实验检测IBP表达对ACC2细胞增殖的影响。结果 IBP在20例正常涎腺中全部表达阳性,在27例涎腺腺样囊性癌中仅3例低表达,余皆不表达,阳性率为11.11%;将IBP转染入ACC2细胞内以后,其增殖明显受到抑制(P<0.05);同时克隆形成实验显示IBP转染组的克隆形成率明显低于空白质粒对照组(分别为48%、92%,P<0.05)。结论 IBP能明显抑制腺样囊性癌细胞的增殖。Objective To investigate the effect of IRF-4 binding protein（IBP） on human salivary adenoid cystic carcinoma（SACC） cell proliferation.Methods IBP expression was detected and analyzed in 27 paraffin-embedded human SACC tissue specimens and 20 paraffin-embedded normal salivary gland tissue specimens with immunohistochemical staining（S-P method）.ACC2 human SACC cells was transfected with plasmid pEGFP-C1/IBP（IBP group） and blank control plasmid pEGFP-C1（control group） via lipofection.Then,the positive cells were screened with G418 to establish a cell strain with stable IBP overexpression.The effect of IBP on ACC2 cell proliferation was studied with MTT method and plate colony formation assay.Results IBP was positively expressed in all normal salivary gland tissue specimens,weakly expressed in 3 human SACC tissue specimens,and not expressed in other 24 SACC tissue specimens（IBP positive rate=11.11% in human SACC tissue specimens）.The proliferation of ACC2 cells in the IBP group was significantly inhibited（P0.05）.In addition,the colony formation assay results indicated that the colony formation rate of the IBP group was significantly lower than that of the control group（48% vs 92%,P0.05）.Conclusion IBP can significantly inhibit SACC cell proliferation.

关 键 词：免疫组化 IRF-4结合蛋白 克隆形成 

分 类 号：R341[医药卫生—基础医学] R730.23